dc.contributor.author | Maloney, Jenny G | |
dc.contributor.author | Molokin, Aleksey | |
dc.contributor.author | Seguí, Raimundo | |
dc.contributor.author | Maravilla, Pablo | |
dc.contributor.author | Martínez-Hernández, Fernando | |
dc.contributor.author | Villalobos, Guiehdani | |
dc.contributor.author | Tsaousis, Anastasios D | |
dc.contributor.author | Gentekaki, Eleni | |
dc.contributor.author | Muñoz-Antolí, Carla | |
dc.contributor.author | Klisiowicz, Debora R | |
dc.contributor.author | Oishi, Camila Y | |
dc.contributor.author | Toledo, Rafael | |
dc.contributor.author | Esteban, J Guillermo | |
dc.contributor.author | Köster, Pamela Carolina | |
dc.contributor.author | Lucio, Aida de | |
dc.contributor.author | Dashti, Alejandro | |
dc.contributor.author | Bailo-Barroso, Begoña | |
dc.contributor.author | Calero-Bernal, Rafael | |
dc.contributor.author | Gonzalez-Barrio, David | |
dc.contributor.author | Carmena, David | |
dc.contributor.author | Santín, Mónica | |
dc.date.accessioned | 2023-07-17T10:35:27Z | |
dc.date.available | 2023-07-17T10:35:27Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Microorganisms. 2023;11(1):46. | es_ES |
dc.identifier.issn | 2076-2607 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16266 | |
dc.description.abstract | Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity. | es_ES |
dc.description.sponsorship | This research was funded by USDA-ARS Project No.: 8042-32000-112-00-D and the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain)-Collaborative Research Network on Tropical Diseases (RICET) and the European Regional Development Fund (ERDF), under project RD12/0018/0013. Additional funding was also provided by the ISCIII under project PI16CIII/00024. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Blastocystis | es_ES |
dc.subject | B. lapemi | es_ES |
dc.subject | Subtype | es_ES |
dc.subject | Oxford Nanopore MinION | es_ES |
dc.subject | ssu rRNA | es_ES |
dc.title | Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38 | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 36677338 | es_ES |
dc.format.volume | 11 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 46 | es_ES |
dc.identifier.doi | 10.3390/microorganisms11010046 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España) | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | United States Department of Agriculture. Agricultural Research Service | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/microorganisms11010046 | es_ES |
dc.identifier.journal | Microorganisms | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/MINECO//RD12%2F0018%2F0013/ES/Enfermedades tropicales/ | es_ES |
dc.relation.projectFIS | info:fis/Instituto de Salud Carlos III/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/Subprograma Estatal de Generación de Conocimiento/ISCIII 2016 Modalidad Proyectos de Investigacion en Salud Intramurales. (2016)/PI16CIII/00024 | es_ES |