Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/16266
Título
Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38
Autor(es)
Maloney, Jenny G | Molokin, Aleksey | Seguí, Raimundo | Maravilla, Pablo | Martínez-Hernández, Fernando | Villalobos, Guiehdani | Tsaousis, Anastasios D | Gentekaki, Eleni | Muñoz-Antolí, Carla | Klisiowicz, Debora R | Oishi, Camila Y | Toledo, Rafael | Esteban, J Guillermo | Köster, Pamela Carolina ISCIII | Lucio, Aida de ISCIII | Dashti, Alejandro ISCIII | Bailo-Barroso, Begoña ISCIII | Calero-Bernal, Rafael | Gonzalez-Barrio, David ISCIII | Carmena, David ISCIII | Santín, Mónica
Fecha de publicación
2023
Cita
Microorganisms. 2023;11(1):46.
Idioma
Inglés
Tipo de documento
research article
Resumen
Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity.
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Versión en línea
DOI
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