Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/16192
Título
A simple method for direct mercury analysis in dried blood spots (DBS) samples for human biomonitoring studies
Autor(es)
González-Rubio, Juana María ISCIII | Domínguez-Morueco, Noelia ISCIII | Pedraza-Diaz, Susana ISCIII | Cañas Portilla, Ana Isabel ISCIII | Lucena-Lozano, Miguel Angel ISCIII | Rodriguez, Ana ISCIII | Castaño, Argelia ISCIII | Esteban-Lopez, Marta ISCIII
Fecha de publicación
2023-07
Cita
Environ Int. 2023 Jul;177:107958.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Human exposure to mercury can have serious health effects, especially in vulnerable groups such as children and fetuses. The use of dried blood spot (DBS) samples to collect capillary blood greatly facilitates sample collection and fieldwork, being a less invasive alternative to blood collection by venipuncture, needing a small volume of sample, and does not require specialized medical staff. Moreover, DBS sampling reduces logistical and financial barriers related to transport and storage of blood samples. We propose here a novel method to analyze total mercury in DBS samples in a Direct Mercury Analyzer (DMA) that allow the control of the volume of the DBS samples. This method has shown good results in terms of precision (<6% error), accuracy (<10% coefficient of variation) and recovery (75-106%). The applicability of the method in human biomonitoring (HBM) was demonstrated in a pilot study involving 41 adults aged 18-65. Mercury concentrations of DBS samples from capillary blood collected by finger prick (real DBS samples) were determined in the DMA and compared with those determined in whole blood (venous blood) by ICP-MS, the method usually used in HBM. The sampling procedure was also validated by comparison of real DBS samples and DBS generated artificially in the laboratory by depositing venous samples in cellulose cards (laboratory DBS). There were no statistically significant differences in the results obtained using both methodologies (DMA: Geometric Mean (confidence interval 95%) = 3.87 (3.12-4.79) µg/L; ICP-MS: Geometric Mean (confidence interval 95%) = 3.46 (2.80-4.27) µg/L). The proposed method is an excellent alternative to be applied in clinical settings as screening methodology for assessing mercury exposure in vulnerable groups, such us pregnant woman, babies and children.
Palabras clave
Direct Mercury Analyser (DMA) | Dried Blood Spot (DBS) | HBM4EU | Human Biomonitoring | Mercury | Microsampling | Non-invasive
MESH
Biological Monitoring | Mercury | Adult | Pregnancy | Female | Child | Humans | Pilot Projects | Blood Specimen Collection
Versión en línea
DOI
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