Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/16143
Título
Identification of small molecules capable of enhancing viral membrane fusion
Autor(es)
Fecha de publicación
2023-05-24
Cita
Virol J. 2023 May 24;20(1):99.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Several approaches have been developed to analyze the entry of highly pathogenic viruses. In this study, we report the implementation of a Bimolecular Multicellular Complementation (BiMuC) assay to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, we screened a library of approved drugs and identified compounds that enhance S protein-mediated cell-cell membrane fusion. Among them, ethynylestradiol promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. Our findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2.
Palabras clave
Enveloped virus | HTS | High-throughput screening | Influenza a virus | Membrane fusion | Nipah virus | SARS-CoV-2 | Syncytium | Viral entry
MESH
DOI
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