dc.contributor.author | García-Murria, Mª Jesús | |
dc.contributor.author | Gadea-Salom, Laura | |
dc.contributor.author | Moreno, Sandra | |
dc.contributor.author | Rius-Salvador, Marina | |
dc.contributor.author | Zaragoza, Oscar | |
dc.contributor.author | Brun, Alejandro | |
dc.contributor.author | Mingarro, Ismael | |
dc.contributor.author | Martínez-Gil, Luis | |
dc.date.accessioned | 2023-06-06T11:07:25Z | |
dc.date.available | 2023-06-06T11:07:25Z | |
dc.date.issued | 2023-05-24 | |
dc.identifier.citation | Virol J. 2023 May 24;20(1):99. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16143 | |
dc.description.abstract | Several approaches have been developed to analyze the entry of highly pathogenic viruses. In this study, we report the implementation of a Bimolecular Multicellular Complementation (BiMuC) assay to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, we screened a library of approved drugs and identified compounds that enhance S protein-mediated cell-cell membrane fusion. Among them, ethynylestradiol promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. Our findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2. | es_ES |
dc.description.sponsorship | This work was supported by the Generalitat Valenciana (PROMETEO/2019/065) and grant PID2020-119111GB-I00 by MCIN/AEI/10.13039/501100011033. O.Z. is funded by grant PID2020-114546RB by MCIN/AEI/10.13039/501100011033. L.G. is the recipient of a predoctoral contract (CIACIF/2021/119) from the Generatitat Valenciana. M.R-S is the recipient of a predoctoral contract from the Spanish Ministry of Science and Innovation (PRE2021-101042 by MCIN/AEI/10.13039/501100011033). This publication was also supported by the European Virus Archive GLOBAL (EVA-GLOBAL) project which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement 871029. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central (BMC) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Enveloped virus | es_ES |
dc.subject | HTS | es_ES |
dc.subject | High-throughput screening | es_ES |
dc.subject | Influenza a virus | es_ES |
dc.subject | Membrane fusion | es_ES |
dc.subject | Nipah virus | es_ES |
dc.subject | SARS-CoV-2 | es_ES |
dc.subject | Syncytium | es_ES |
dc.subject | Viral entry | es_ES |
dc.subject.mesh | COVID-19 | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | SARS-CoV-2 | es_ES |
dc.subject.mesh | Virus Internalization | es_ES |
dc.subject.mesh | Biological Assay | es_ES |
dc.subject.mesh | Gene Library | es_ES |
dc.title | Identification of small molecules capable of enhancing viral membrane fusion | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 37226231 | es_ES |
dc.format.volume | 20 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 99 | es_ES |
dc.identifier.doi | 10.1186/s12985-023-02068-1 | es_ES |
dc.contributor.funder | Generalitat Valenciana (España) | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. H2020 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1743-422X | es_ES |
dc.identifier.journal | Virology journal | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/871029/EU | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2020-119111GB-I00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2020-114546RB | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PRE2021-101042 | es_ES |