Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/15287
Title
Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4+ T Cells From HIV-1 Patients
Author(s)
Date issued
2022-04
Citation
Front Virol. 2022 Apr;2:869431.
Language
Inglés
Document type
journal article
Abstract
We described a novel HIV autologous isolation method based in coculturing macrophages and CD4+T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 1010HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials.
Subject
CD4+ T cells | HIV isolation | HIV therapeutic vaccine | Autologous HIV immunogen | Coculture | Monocyte-derived macrophages (MDM)
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