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dc.contributor.authorMontes-Casado, Maria 
dc.contributor.authorSanvicente, Adrian
dc.contributor.authorCasarrubios, Laura
dc.contributor.authorFeito, María José
dc.contributor.authorRojo, José M
dc.contributor.authorVallet-Regí, María
dc.contributor.authorArcos, Daniel
dc.contributor.authorPortoles, Pilar 
dc.contributor.authorPortolés, María Teresa
dc.date.accessioned2021-04-24T09:58:17Z
dc.date.available2021-04-24T09:58:17Z
dc.date.issued2020-11-05
dc.identifier.citationInt J Mol Sci . 2020 Nov 5;21(21):8291.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12772
dc.description.abstractMesoporous bioactive glass nanospheres (NanoMBGs) have high potential for clinical applications. However, the impact of these nanoparticles on the immune system needs to be addressed. In this study, the biocompatibility of SiO2-CaO NanoMBGs was evaluated on different mouse immune cells, including spleen cells subsets, bone marrow-derived dendritic cells (BMDCs), or cell lines like SR.D10 Th2 CD4+ lymphocytes and DC2.4 dendritic cells. Flow cytometry and confocal microscopy show that the nanoparticles were rapidly and efficiently taken up in vitro by T and B lymphocytes or by specialized antigen-presenting cells (APCs) like dendritic cells (DCs). Nanoparticles were not cytotoxic and had no effect on cell viability or proliferation under T-cell (anti-CD3) or B cell (LPS) stimuli. Besides, NanoMBGs did not affect the balance of spleen cell subsets, or the production of intracellular or secreted pro- and anti-inflammatory cytokines (TNF-α, IFN-γ, IL-2, IL-6, IL-10) by activated T, B, and dendritic cells (DC), as determined by flow cytometry and ELISA. T cell activation surface markers (CD25, CD69 and Induced Costimulator, ICOS) were not altered by NanoMBGs. Maturation of BMDCs or DC2.4 cells in vitro was not altered by NanoMBGs, as shown by expression of Major Histocompatibility Complex (MHC) and costimulatory molecules (CD40, CD80, CD86), or IL-6 secretion. The effect of wortmannin and chlorpromazine indicate a role for phosphoinositide 3-kinase (PI3K), actin and clathrin-dependent pathways in NanoMBG internalization. We thus demonstrate that these NanoMBGs are both non-toxic and non-inflammagenic for murine lymphoid cells and myeloid DCs despite their efficient intake by the cells.es_ES
dc.description.sponsorshipThis research was funded by Acción Estratégica en Salud Intramural (AESI) del Instituto de Salud Carlos III (AESI PI16CIII/00012 to P.P.); Ministerio de Economía y Competitividad, Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) (MAT2016-75611-R AEI/FEDER, UE to D.A. and M.T.P.). M.V.R acknowledges funding from the European Research Council (Advanced Grant VERDI; ERC-2015-AdG Proposal No. 694160). A.S. acknowledges the financial support from Comunidad de Madrid (Spain, EJD-2018-PRE/BMD-8558). L.C. is grateful to the Universidad Complutense de Madrid for a UCM fellowship.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectB celles_ES
dc.subjectICOSes_ES
dc.subjectT celles_ES
dc.subjectDendritic celles_ES
dc.subjectDendritic cell maturationes_ES
dc.subjectEndocytosises_ES
dc.subjectMesoporous bioactive glasses_ES
dc.subjectNanomateriales_ES
dc.subjectPhosphoinositide 3-kinase PI3-Kes_ES
dc.subject.meshAnimals es_ES
dc.subject.meshBone Marrow Cells es_ES
dc.subject.meshCalcium Compounds es_ES
dc.subject.meshCell Differentiation es_ES
dc.subject.meshCell Proliferation es_ES
dc.subject.meshCell Survival es_ES
dc.subject.meshCells, Cultured es_ES
dc.subject.meshCytokines es_ES
dc.subject.meshDendritic Cells es_ES
dc.subject.meshFemale es_ES
dc.subject.meshImmunologic Techniques es_ES
dc.subject.meshInflammation Mediators es_ES
dc.subject.meshLymphocyte Activation es_ES
dc.subject.meshMale es_ES
dc.subject.meshMaterials Testing es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred C57BL es_ES
dc.subject.meshNanospheres es_ES
dc.subject.meshOxides es_ES
dc.subject.meshPorosity es_ES
dc.subject.meshSilicon Dioxide es_ES
dc.subject.meshSpleen es_ES
dc.titleAn Immunological Approach to the Biocompatibility of Mesoporous SiO2-CaO Nanospheres.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID33167415es_ES
dc.format.volume21es_ES
dc.format.number21es_ES
dc.identifier.doi10.3390/ijms21218291es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderAgencia Estatal de Investigación (España) 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC) 
dc.contributor.funderComunidad de Madrid (España) 
dc.contributor.funderComplutense University of Madrid (España) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1422-0067es_ES
dc.relation.publisherversionhttps://doi.org/10.3390/ijms21218291es_ES
dc.identifier.journalInternational Journal of Molecular Scienceses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI16CIII/00012es_ES
dc.rights.accessRightsopen accesses_ES


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