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dc.contributor.author | Montes-Casado, Maria | |
dc.contributor.author | Sanvicente, Adrian | |
dc.contributor.author | Casarrubios, Laura | |
dc.contributor.author | Feito, María José | |
dc.contributor.author | Rojo, José M | |
dc.contributor.author | Vallet-Regí, María | |
dc.contributor.author | Arcos, Daniel | |
dc.contributor.author | Portoles, Pilar | |
dc.contributor.author | Portolés, María Teresa | |
dc.date.accessioned | 2021-04-24T09:58:17Z | |
dc.date.available | 2021-04-24T09:58:17Z | |
dc.date.issued | 2020-11-05 | |
dc.identifier.citation | Int J Mol Sci . 2020 Nov 5;21(21):8291. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/12772 | |
dc.description.abstract | Mesoporous bioactive glass nanospheres (NanoMBGs) have high potential for clinical applications. However, the impact of these nanoparticles on the immune system needs to be addressed. In this study, the biocompatibility of SiO2-CaO NanoMBGs was evaluated on different mouse immune cells, including spleen cells subsets, bone marrow-derived dendritic cells (BMDCs), or cell lines like SR.D10 Th2 CD4+ lymphocytes and DC2.4 dendritic cells. Flow cytometry and confocal microscopy show that the nanoparticles were rapidly and efficiently taken up in vitro by T and B lymphocytes or by specialized antigen-presenting cells (APCs) like dendritic cells (DCs). Nanoparticles were not cytotoxic and had no effect on cell viability or proliferation under T-cell (anti-CD3) or B cell (LPS) stimuli. Besides, NanoMBGs did not affect the balance of spleen cell subsets, or the production of intracellular or secreted pro- and anti-inflammatory cytokines (TNF-α, IFN-γ, IL-2, IL-6, IL-10) by activated T, B, and dendritic cells (DC), as determined by flow cytometry and ELISA. T cell activation surface markers (CD25, CD69 and Induced Costimulator, ICOS) were not altered by NanoMBGs. Maturation of BMDCs or DC2.4 cells in vitro was not altered by NanoMBGs, as shown by expression of Major Histocompatibility Complex (MHC) and costimulatory molecules (CD40, CD80, CD86), or IL-6 secretion. The effect of wortmannin and chlorpromazine indicate a role for phosphoinositide 3-kinase (PI3K), actin and clathrin-dependent pathways in NanoMBG internalization. We thus demonstrate that these NanoMBGs are both non-toxic and non-inflammagenic for murine lymphoid cells and myeloid DCs despite their efficient intake by the cells. | es_ES |
dc.description.sponsorship | This research was funded by Acción Estratégica en Salud Intramural (AESI) del Instituto de Salud Carlos III (AESI PI16CIII/00012 to P.P.); Ministerio de Economía y Competitividad, Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) (MAT2016-75611-R AEI/FEDER, UE to D.A. and M.T.P.). M.V.R acknowledges funding from the European Research Council (Advanced Grant VERDI; ERC-2015-AdG Proposal No. 694160). A.S. acknowledges the financial support from Comunidad de Madrid (Spain, EJD-2018-PRE/BMD-8558). L.C. is grateful to the Universidad Complutense de Madrid for a UCM fellowship. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | B cell | es_ES |
dc.subject | ICOS | es_ES |
dc.subject | T cell | es_ES |
dc.subject | Dendritic cell | es_ES |
dc.subject | Dendritic cell maturation | es_ES |
dc.subject | Endocytosis | es_ES |
dc.subject | Mesoporous bioactive glass | es_ES |
dc.subject | Nanomaterial | es_ES |
dc.subject | Phosphoinositide 3-kinase PI3-K | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Bone Marrow Cells | es_ES |
dc.subject.mesh | Calcium Compounds | es_ES |
dc.subject.mesh | Cell Differentiation | es_ES |
dc.subject.mesh | Cell Proliferation | es_ES |
dc.subject.mesh | Cell Survival | es_ES |
dc.subject.mesh | Cells, Cultured | es_ES |
dc.subject.mesh | Cytokines | es_ES |
dc.subject.mesh | Dendritic Cells | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Immunologic Techniques | es_ES |
dc.subject.mesh | Inflammation Mediators | es_ES |
dc.subject.mesh | Lymphocyte Activation | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Materials Testing | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mice, Inbred C57BL | es_ES |
dc.subject.mesh | Nanospheres | es_ES |
dc.subject.mesh | Oxides | es_ES |
dc.subject.mesh | Porosity | es_ES |
dc.subject.mesh | Silicon Dioxide | es_ES |
dc.subject.mesh | Spleen | es_ES |
dc.title | An Immunological Approach to the Biocompatibility of Mesoporous SiO2-CaO Nanospheres. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 33167415 | es_ES |
dc.format.volume | 21 | es_ES |
dc.format.number | 21 | es_ES |
dc.identifier.doi | 10.3390/ijms21218291 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Agencia Estatal de Investigación (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.contributor.funder | Unión Europea. Comisión Europea. European Research Council (ERC) | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | Complutense University of Madrid (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1422-0067 | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/ijms21218291 | es_ES |
dc.identifier.journal | International Journal of Molecular Sciences | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PI16CIII/00012 | es_ES |
dc.rights.accessRights | open access | es_ES |