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Título
Multilocus Genotyping of Giardia duodenalis in Mostly Asymptomatic Indigenous People from the Tapirapé Tribe, Brazilian Amazon.
Autor(es)
Köster, Pamela Carolina ISCIII | Malheiros, Antonio F | Shaw, Jeffrey J | Balasegaram, Sooria | Prendergast, Alexander | Lucaccioni, Héloïse | Moreira, Luciana Melhorança | Lemos, Larissa M S | Dashti, Alejandro ISCIII | Bailo-Barroso, Begoña ISCIII | Marcili, Arlei | Sousa Soares, Herbert | Gennari, Solange Maria | Calero-Bernal, Rafael | Gonzalez-Barrio, David ISCIII | Carmena, David ISCIII
Fecha de publicación
2021-02-14
Cita
Pathogens . 2021 Feb 14;10(2):206.
Idioma
Inglés
Tipo de documento
research article
Resumen
Little information is available on the occurrence and genetic variability of the diarrhoea-causing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey describes the frequency, genotypes, and risk associations for this pathogen in Tapirapé people (Brazilian Amazon) at four sampling campaigns during 2008-2009. Microscopy was used as a screening test, and molecular (PCR and Sanger sequencing) assays targeting the small subunit ribosomal RNA, the glutamate dehydrogenase, the beta-giardin, and the triosephosphate isomerase genes as confirmatory/genotyping methods. Associations between G. duodenalis and sociodemographic and clinical variables were investigated using Chi-squared test and univariable/multivariable logistic regression models. Overall, 574 individuals belonging to six tribes participated in the study, with G. duodenalis prevalence rates varying from 13.5-21.7%. The infection was positively linked to younger age and tribe. Infected children <15 years old reported more frequent gastrointestinal symptoms compared to adults. Assemblage B accounted for three out of four G. duodenalis infections and showed a high genetic diversity. No association between assemblage and age or occurrence of diarrhoea was demonstrated. These data indicate that the most likely source of infection was anthropic and that different pathways (e.g., drinking water) may be involved in the transmission of the parasite.
Palabras clave
Amazon | Brazil | Giardia | Tapirapé | Asymptomatic | Community | Genotyping | Indigenous | Risk association | Transmission
Versión en línea
DOI
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