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dc.contributor.authorValdezate, Sylvia 
dc.contributor.authorCobo, Fernando
dc.contributor.authorMonzón, Sara
dc.contributor.authorMedina-Pascual, Maria Jose 
dc.contributor.authorZaballos, Angel 
dc.contributor.authorCuesta, Isabel 
dc.contributor.authorPino-Rosa, Silvia del
dc.contributor.authorVillalon-Panzano, Pilar 
dc.date.accessioned2021-04-08T11:31:43Z
dc.date.available2021-04-08T11:31:43Z
dc.date.issued2021-03-16
dc.identifier.citationAntibiotics (Basel). 2021 Mar 16;10(3):304.es_ES
dc.identifier.issn2079-6382es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12561
dc.description.abstractBackground: Bacteroides fragilis shows high antimicrobial resistance (AMR) rates and possesses numerous AMR mechanisms. Its carbapenem-resistant strains (metallo-β-lactamase cfiApositive) appear as an emergent, evolving clade. Methods: This work examines the genomes, taxonomy, and phylogenetic relationships with respect to other B. fragilis genomes of two B. fragilis strains (CNM20180471 and CNM20200206) resistant to meropenem+EDTA and other antimicrobial agents. Results: Both strains possessed cfiA genes (cfiA14b and the new cfiA28), along with other AMR mechanisms. The presence of other efflux-pump genes, mexAB/mexJK/mexXY-oprM, acrEF/mdtEFtolC, and especially cusR, which reduces the entry of carbapenem via the repression of porin OprD, may be related to meropenem–EDTA resistance. None of the detected insertion sequences were located upstream of cfiA. The genomes of these and other B. fragilis strains that clustered together in phylogenetic analyses did not meet the condition of >95% average nucleotide/amino acid identity, or >70% in silico genome-to-genome hybridization similarity, to be deemed members of the same species, although <1% difference in the genomic G+C content was seen with respect to the reference genome B. fragilis NCTC 9343T. Conclusions: Carbapenem-resistant strains may be considered a distinct clonal entity, and their surveillance is recommended given the ease with which they appear to acquire AMR.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBacteroides fragilises_ES
dc.subjectCarbapenemses_ES
dc.subjectcfiA14es_ES
dc.subjectcfiA28es_ES
dc.subjectDivision IIes_ES
dc.subjectPorin inactivationes_ES
dc.subjectSusceptibilityes_ES
dc.titleGenomic Background and Phylogeny of cfiA-Positive Bacteroides fragilis Strains Resistant to Meropenem-EDTAes_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID33809460es_ES
dc.format.volume10es_ES
dc.format.number3es_ES
dc.identifier.doi10.3390/antibiotics10030304es_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/antibiotics10030304es_ES
dc.identifier.journalAntibiotics (Basel, Switzerland)es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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