Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/11124
Título
Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish.
Autor(es)
García-Poyatos, Carolina | Cogliati, Sara CNIC | Calvo, Enrique CNIC | Hernansanz-Agustin, Pablo | Lagarrigue, Sylviane | Magni, Ricardo CNIC | Botos, Marius | Langa, Xavier | Amati, Francesca | Vazquez, Jesus CNIC | Mercader, Nadia CNIC | Enriquez, Jose Antonio CNIC
Fecha de publicación
2020-07-03
Cita
EMBO Rep. 2020; 21(7):e50287
Idioma
Inglés
Tipo de documento
journal article
Resumen
The oxidative phosphorylation (OXPHOS) system is a dynamic system in which the respiratory complexes coexist with super-assembled quaternary structures called supercomplexes (SCs). The physiological role of SCs is still disputed. Here, we used zebrafish to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to characterize these structures and found similar SCs to those described in mice, as well as novel SCs including III2 + IV2 , I + IV, and I + III2 + IV2 . To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly factor 1 (scaf1). scaf1-/- fish displayed altered OXPHOS activity due to the disrupted interaction of complexes III and IV. scaf1-/- fish were smaller in size and showed abnormal fat deposition and decreased female fertility. These physiological phenotypes were rescued by doubling the food supply, which correlated with improved bioenergetics and alterations in the metabolic gene expression program. These results reveal that SC assembly by Scaf1 modulates OXPHOS efficiency and allows the optimization of metabolic resources.
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DOI
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