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dc.contributor.authorGarcía-Poyatos, Carolina
dc.contributor.authorCogliati, Sara 
dc.contributor.authorCalvo, Enrique 
dc.contributor.authorHernansanz-Agustín, Pablo
dc.contributor.authorLagarrigue, Sylviane
dc.contributor.authorMagni, Ricardo 
dc.contributor.authorBotos, Marius
dc.contributor.authorLanga, Xavier
dc.contributor.authorAmati, Francesca
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorMercader, Nadia 
dc.contributor.authorEnriquez, José Antonio
dc.identifier.citationEMBO Rep. 2020; 21(7):e50287es_ES
dc.description.abstractThe oxidative phosphorylation (OXPHOS) system is a dynamic system in which the respiratory complexes coexist with super-assembled quaternary structures called supercomplexes (SCs). The physiological role of SCs is still disputed. Here, we used zebrafish to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to characterize these structures and found similar SCs to those described in mice, as well as novel SCs including III2  + IV2 , I + IV, and I + III2  + IV2 . To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly factor 1 (scaf1). scaf1-/- fish displayed altered OXPHOS activity due to the disrupted interaction of complexes III and IV. scaf1-/- fish were smaller in size and showed abnormal fat deposition and decreased female fertility. These physiological phenotypes were rescued by doubling the food supply, which correlated with improved bioenergetics and alterations in the metabolic gene expression program. These results reveal that SC assembly by Scaf1 modulates OXPHOS efficiency and allows the optimization of metabolic resources.es_ES
dc.description.sponsorshipA.E. was supported by Spanish Ministry of Economy and Competitiveness, MINECO (SAF2015-65633-R), CIBERFES (CB16/10/00282), and HFSP (RGP0016/2018). N.M. was funded by the ERC starting grant 337703, HFSP (RGP0016/2018), and SNF 31003A-159721. FA was funded by Swiss National Science Foundation grant 320030_170062. J.V. was funded by MINECO (BIO2015-67580-P), by Carlos III Institute of Health-Fondo de Investigación Sanitaria (PRB3, IPT17/0019 - ISCIIISGEFI/ERDF, ProteoRed), the Fundación La Marato TV3, and by “La Caixa” Banking Foundation (HR17-00247). The CNIC is supported by the Ministry of Economy, Industry and Competitiveness (MEIC) and the Pro-CNIC Foundation and is a Severo Ochoa Center of Excellence (MEIC award SEV-2015-0505).es_ES
dc.publisherEMBO Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleScaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish.es_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Research Counciles_ES
dc.contributor.funderSwiss National Science Foundationes_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)es_ES
dc.contributor.funderFundació La Maratóes_ES
dc.contributor.funderFundación La Caixaes_ES
dc.contributor.funderFundación ProCNICes_ES
dc.identifier.journalEMBO reportses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Genética Funcional del Sistema de Fosforilación Oxidativaes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Desarrollo del Epicardio y su Papel en la Regeneraciónes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES

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