dc.contributor.author | Raro, Otávio Hallal Ferreira | |
dc.contributor.author | da Silva, Ravena Maya Cardoso | |
dc.contributor.author | Filho, Edison Moraes Rodrigues | |
dc.contributor.author | Sukiennik, Teresa Cristina Teixeira | |
dc.contributor.author | Stadnik, Claudio | |
dc.contributor.author | Dias, Cícero Armídio Gomes | |
dc.contributor.author | Oteo-Iglesias, Jesus | |
dc.contributor.author | Perez-Vazquez, Maria | |
dc.date.accessioned | 2020-09-22T10:41:25Z | |
dc.date.available | 2020-09-22T10:41:25Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Front Microbiol . 2020 Jul 15;11:1563. | es_ES |
dc.identifier.issn | 1664-302X | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/11066 | |
dc.description.abstract | Carbapenemase-producing Klebsiella pneumoniae (CP-Kp) is a major cause of infections in transplanted patients and has been associated with high mortality rates in this group. There is a lack of information about the Brazilian structure population of CP-Kp isolated from transplanted patients. By whole-genome sequencing (WGS), we analyzed phylogeny, resistome, virulome of CP-Kp isolates, and the structure of plasmids encoding blaKPC-2 and blaNDM-1 genes.
One K. pneumoniae isolated from each selected transplanted patient colonized or infected by CP-Kp over a 16-month period in a hospital complex in Porto Alegre (Brazil) was submitted for WGS. The total number of strains sequenced was 80. The hospital complex in Porto Alegre comprised seven different hospitals. High-resolution SNP typing, core genome multilocus sequence typing (cgMLST), resistance and virulence genes inference, and plasmid reconstruction were performed in 80 CP-Kp.
The mortality rate of CP-Kp colonized or infected transplanted inpatients was 21.3% (17/80). Four CP-Kp epidemic clones were described: ST11/KPC-2, ST16/KPC-2, and ST15/NDM-1, all responsible for interhospital outbreaks; and ST437/KPC-2 affecting a single hospital. The average number of acquired resistance and virulence genes was 9 (range = 2-14) and 27 (range = 6-36), respectively. Two plasmids carrying the blaKPC-2 were constructed and belonged to IncN and IncM types. Additionally, an IncFIB plasmid carrying the blaNDM-1 was described.
We detected intrahospital and interhospital spread of mobile structures and international K. pneumoniae clones as ST11, ST16, and ST15 among transplanted patients, which carry a significant range of acquired resistance and virulence genes and keep spreading across the world. | es_ES |
dc.description.sponsorship | This work was supported by Plan Nacional de I+D+i 2013–2016 and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Ciencia, Innovación y Universidades, and Spanish Network for Research in Infectious Diseases (REIPI RD16CIII/0004/0002), and co-financed by the European Regional Development Fund ERDF “A way to achieve Europe,” Operative Program Intelligent Growth 2014–2020. This work was also supported in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES) – Finance Code 001. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Transplanted patients | en |
dc.subject | blaKPC−2 | en |
dc.subject | blaNDM−1 | en |
dc.subject | Whole-genome sequencing | en |
dc.subject | cgMLST | en |
dc.subject | Epidemic clones | |
dc.title | Carbapenemase-Producing Klebsiella pneumoniae From Transplanted Patients in Brazil: Phylogeny, Resistome, Virulome and Mobile Genetic Elements Harboring blaKPC-2 or blaNDM-1. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 32760368 | es_ES |
dc.format.volume | 11 | es_ES |
dc.format.page | 1563 | es_ES |
dc.identifier.doi | 10.3389/fmicb.2020.01563 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1664-302X | es_ES |
dc.relation.publisherversion | https://doi.org/10.3389/fmicb.2020.01563 | es_ES |
dc.identifier.journal | Frontiers in microbiology | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/REIPI RD16CIII/0004/0002 | es_ES |
dc.rights.accessRights | open access | es_ES |