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dc.contributor.author | Martinez-Gonzalez, Elena | |
dc.contributor.author | Martín-Carbonero, Luz | |
dc.contributor.author | Brochado-Kith, Oscar | |
dc.contributor.author | Gomez-Sanz, Alicia | |
dc.contributor.author | Jimenez-Sousa, Maria Angeles | |
dc.contributor.author | Martinez-Roman, Paula | |
dc.contributor.author | Resino, Salvador | |
dc.contributor.author | Briz, Veronica | |
dc.contributor.author | Fernandez-Rodriguez, Amanda | |
dc.date.accessioned | 2020-09-14T07:20:14Z | |
dc.date.available | 2020-09-14T07:20:14Z | |
dc.date.issued | 2020-07-07 | |
dc.identifier.citation | Sci Rep . 2020 Jul 7;10(1):11140. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/11002 | |
dc.description.abstract | Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) hijack the host exosomal machinery as an additional mechanism of infection and evasion of the immune system, modifying the small RNA (smRNA) cargo during infection. We characterized the surface epitopes of extracellular vesicles (EVs) from plasma HIV/HCV-coinfected patients and their smRNA cargo profile, by comparing different isolation procedures. Six EVs isolation procedures were compared: ultracentrifugation, and five different polyethylene glycol-based methods (commercial, combined with a column purification step and two custom); and two RNA commercial kits (phenol and non-phenol based) were used. High-throughput sequencing of smRNAs was performed. Exosomal surface epitopes were analyzed by the MACSPlex Exosome Kit. Four miRNAs displayed differences among protocols (hsa-miR-205-5p and hsa-let-7a/b/f-5p). The selection of RNA isolation kit impacted on the detection of miRNAs and other smRNAs, where the phenol-based RNA isolation kit performed acceptably. EVs surface was enriched with HLA-DR/DP/DQ, CD81, and CD8. There were three liver-specific miRNAs overexpressed (let-7a-5p, miR-21-5p and hsa-miR-122-5p), thus, EVs cargo might reflect liver disease evolution. Other smRNAs such as piwi-interacting RNAs were also detected for the first time. Custom polyethylene glycol precipitation-based methods combined with an RNA phenol-based kit yielded the higher number of smRNAs for EVs isolated from plasma HIV/HCV patients. | es_ES |
dc.description.sponsorship | We want to particularly acknowledge the patients in this study for their participation and to the nursery team for the generous participation in collecting clinical samples used in this work. We want also to thank the Bioinformatics Unit at the Institute of Health Carlos III for their valuable support for the bioinformatics analysis. This study was supported by grants from Institute of Health Carlos III (ISCIII; grant numbers CP14CIII/00010 financed to AFR and PI15CIII/00031 and PI18CIII/00020 financed to AFR and VB). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Coinfection | es_ES |
dc.subject.mesh | Exosomes | es_ES |
dc.subject.mesh | Extracellular Vesicles | es_ES |
dc.subject.mesh | HIV Infections | es_ES |
dc.subject.mesh | Hepatitis C | es_ES |
dc.subject.mesh | Female | |
dc.subject.mesh | High-Throughput Nucleotide Sequencing | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Male | |
dc.subject.mesh | MicroRNAs | |
dc.subject.mesh | Middle Aged | |
dc.subject.mesh | RNA, Viral | |
dc.subject.mesh | Real-Time Polymerase Chain Reaction | |
dc.title | Comparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 32636456 | es_ES |
dc.format.volume | 10 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 11140 | es_ES |
dc.identifier.doi | 10.1038/s41598-020-67935-1 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2045-2322 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-020-67935-1 | es_ES |
dc.identifier.journal | Scientific reports | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/CP14CIII/00010 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/PI15CIII/00031 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/PI18CIII/00020 | es_ES |
dc.rights.accessRights | open access | es_ES |