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dc.contributor.authorMartinez-Gonzalez, Elena 
dc.contributor.authorMartín-Carbonero, Luz
dc.contributor.authorBrochado-Kith, Oscar 
dc.contributor.authorGomez-Sanz, Alicia 
dc.contributor.authorJimenez-Sousa, Maria Angeles 
dc.contributor.authorMartinez-Roman, Paula 
dc.contributor.authorResino, Salvador 
dc.contributor.authorBriz, Veronica 
dc.contributor.authorFernandez-Rodriguez, Amanda 
dc.date.accessioned2020-09-14T07:20:14Z
dc.date.available2020-09-14T07:20:14Z
dc.date.issued2020-07-07
dc.identifier.citationSci Rep . 2020 Jul 7;10(1):11140.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/11002
dc.description.abstractHepatitis C virus (HCV) and human immunodeficiency virus (HIV) hijack the host exosomal machinery as an additional mechanism of infection and evasion of the immune system, modifying the small RNA (smRNA) cargo during infection. We characterized the surface epitopes of extracellular vesicles (EVs) from plasma HIV/HCV-coinfected patients and their smRNA cargo profile, by comparing different isolation procedures. Six EVs isolation procedures were compared: ultracentrifugation, and five different polyethylene glycol-based methods (commercial, combined with a column purification step and two custom); and two RNA commercial kits (phenol and non-phenol based) were used. High-throughput sequencing of smRNAs was performed. Exosomal surface epitopes were analyzed by the MACSPlex Exosome Kit. Four miRNAs displayed differences among protocols (hsa-miR-205-5p and hsa-let-7a/b/f-5p). The selection of RNA isolation kit impacted on the detection of miRNAs and other smRNAs, where the phenol-based RNA isolation kit performed acceptably. EVs surface was enriched with HLA-DR/DP/DQ, CD81, and CD8. There were three liver-specific miRNAs overexpressed (let-7a-5p, miR-21-5p and hsa-miR-122-5p), thus, EVs cargo might reflect liver disease evolution. Other smRNAs such as piwi-interacting RNAs were also detected for the first time. Custom polyethylene glycol precipitation-based methods combined with an RNA phenol-based kit yielded the higher number of smRNAs for EVs isolated from plasma HIV/HCV patients.es_ES
dc.description.sponsorshipWe want to particularly acknowledge the patients in this study for their participation and to the nursery team for the generous participation in collecting clinical samples used in this work. We want also to thank the Bioinformatics Unit at the Institute of Health Carlos III for their valuable support for the bioinformatics analysis. This study was supported by grants from Institute of Health Carlos III (ISCIII; grant numbers CP14CIII/00010 financed to AFR and PI15CIII/00031 and PI18CIII/00020 financed to AFR and VB).es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Group es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshCoinfection es_ES
dc.subject.meshExosomes es_ES
dc.subject.meshExtracellular Vesicles es_ES
dc.subject.meshHIV Infections es_ES
dc.subject.meshHepatitis C es_ES
dc.subject.meshFemale 
dc.subject.meshHigh-Throughput Nucleotide Sequencing 
dc.subject.meshHumans 
dc.subject.meshMale 
dc.subject.meshMicroRNAs 
dc.subject.meshMiddle Aged 
dc.subject.meshRNA, Viral 
dc.subject.meshReal-Time Polymerase Chain Reaction 
dc.titleComparison of methods and characterization of small RNAs from plasma extracellular vesicles of HIV/HCV coinfected patients.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID32636456es_ES
dc.format.volume10es_ES
dc.format.number1es_ES
dc.format.page11140es_ES
dc.identifier.doi10.1038/s41598-020-67935-1es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.description.peerreviewedes_ES
dc.identifier.e-issn2045-2322es_ES
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-020-67935-1es_ES
dc.identifier.journalScientific reportses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/CP14CIII/00010es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI15CIII/00031es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI18CIII/00020es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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