Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/10455
Evidence of ongoing replication in a human immunodeficiency virus type 1 persistently infected cell line.
Sánchez-Jiménez, Carmen | Toledano, Víctor | Lopez-Galindez, Luis Cecilio ISCIII | Olivares, Isabel ISCIII | Reis Vieira, Catarina CNIC | Gutiirrez-Rivas, Monica ISCIII | López, C
J Gen Virol . 2013 May;94(Pt 5):944-954
Human immunodeficiency virus type 1 (HIV-1) persistently infected cell lines are characterized by the continuous viral production without cytopathic effect. However, it is not completely clear if this production is contributed only by viral transcription or also by new cycles of viral replication. We studied an HIV-1 persistently infected cell line, designated H61-D, providing evidence of new replication cycles as sustained by: (i) a decrease in viral production, measured by p24 protein, after treatment of the culture with 3'-azydo-3'-deoxythymydine; (ii) detection of new integration events in the course of cell culture, and (iii) finding of two-long-terminal repeat circles in the cells. H61-D cells were not infected by cell-free virus, but infection was possible by co-culture with another productive-infected cell line. In conclusion, ongoing viral replication is taking place in H61-D persistent cells and new infections are mediated by a cell-to-cell spread mechanism.
Virus Replication | Anti-HIV Agents | Cell Line | Coculture Techniques | DNA, Viral | HIV Core Protein p24 | HIV Infections | HIV-1 | Humans | Virus Integration | Zidovudine
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