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dc.contributor.authorSánchez-Jiménez, Carmen
dc.contributor.authorToledano, Víctor
dc.contributor.authorLopez-Galindez, Luis Cecilio 
dc.contributor.authorOlivares, Isabel 
dc.contributor.authorReis Vieira, Catarina 
dc.contributor.authorGutiérrez-Rivas, Mónica 
dc.contributor.authorLópez, C
dc.date.accessioned2020-06-16T08:48:48Z
dc.date.available2020-06-16T08:48:48Z
dc.date.issued2013-05
dc.identifier.citationJ Gen Virol . 2013 May;94(Pt 5):944-954es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10455
dc.description.abstractHuman immunodeficiency virus type 1 (HIV-1) persistently infected cell lines are characterized by the continuous viral production without cytopathic effect. However, it is not completely clear if this production is contributed only by viral transcription or also by new cycles of viral replication. We studied an HIV-1 persistently infected cell line, designated H61-D, providing evidence of new replication cycles as sustained by: (i) a decrease in viral production, measured by p24 protein, after treatment of the culture with 3'-azydo-3'-deoxythymydine; (ii) detection of new integration events in the course of cell culture, and (iii) finding of two-long-terminal repeat circles in the cells. H61-D cells were not infected by cell-free virus, but infection was possible by co-culture with another productive-infected cell line. In conclusion, ongoing viral replication is taking place in H61-D persistent cells and new infections are mediated by a cell-to-cell spread mechanism.es_ES
dc.description.sponsorshipWork in CNM is supported by grant SAF 2007-61036and 2010-17226 from MICINN Spain, by grants 36558/06, 36641/07,36779/08, 360766/09 from FIPSE Spain, and in part by the RETIC deInvestigacio ́n en SIDA (Red de grupos 173) of the Fondo deInvestigaciones Sanitarias (FIS).es_ES
dc.language.isoenges_ES
dc.publisherMicrobiology Society es_ES
dc.relation.isversionofPostprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshVirus Replication es_ES
dc.subject.meshAnti-HIV Agents es_ES
dc.subject.meshCell Line es_ES
dc.subject.meshCoculture Techniques es_ES
dc.subject.meshDNA, Viral es_ES
dc.subject.meshHIV Core Protein p24 es_ES
dc.subject.meshHIV Infections es_ES
dc.subject.meshHIV-1 es_ES
dc.subject.meshHumans es_ES
dc.subject.meshVirus Integration es_ES
dc.subject.meshZidovudine es_ES
dc.titleEvidence of ongoing replication in a human immunodeficiency virus type 1 persistently infected cell line.es_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID23288426es_ES
dc.format.volume94es_ES
dc.format.numberPt 5es_ES
dc.format.page944-954es_ES
dc.identifier.doi10.1099/vir.0.046573-0es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.contributor.funderRed Española de Investigación en SIDA
dc.contributor.funderInstituto de Salud Carlos III 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1465-2099es_ES
dc.relation.publisherversionhttps://doi.org/10.1099/vir.0.046573-0es_ES
dc.identifier.journalThe Journal of general virologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/SAF 2007-61036es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/ 2010-17226es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/36558/06es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/36641/07es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/36779/08es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/360766/09es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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