Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/10287
Interleukin-2 as a marker for detecting asymptomatic individuals in areas where Leishmania infantum is endemic.
Ibarra-Meneses, Ana Victoria ISCIII | Carrillo, Eugenia ISCIII | Sánchez, C | García-Martínez, J | López Lacomba, D | San Martín, Juan Víctor | Alves, F | Alvar, Jorge ISCIII | Moreno, Javier ISCIII
Clin Microbiol Infect . 2016 Aug;22(8):739.e1-4
No field method exists for identifying asymptomatic individuals in areas where Leishmania infantum is endemic. This work reports that, 24 h after stimulating whole blood with soluble Leishmania antigen (SLA), plasma interferon-γ (IFN-γ) and interleukin-2 (IL-2) become significantly elevated in samples from asymptomatic individuals (n=47) compared with those from negative controls (n=50), all of them recruited from a blood bank. When compared with the reference test SLA-lymphoproliferative assay, IL-2 appears as a new, 100% sensitive and specific marker for asymptomatic individuals with a positive cellular response (compared with 100% and 84.78%, respectively, for IFN-γ). Further studies in other transmission areas and in other cohorts of exposed people need to be performed to confirm these results. Once validated, IFN-γ and IL-2 levels in SLA-stimulated whole blood could be reliably used in the field to estimate the prevalence of those asymptomatic individuals with Leishmania-specific cellular immune responses.
Asymptomatic Diseases | Leishmania infantum | Adult | Biomarkers | Cytokines | Female | Humans | Interleukin-2 | Leishmaniasis, Visceral | Male | Middle Aged | Young Adult
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