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dc.contributor.authorMartin-Martin, Ines 
dc.contributor.authorMolina, Ricardo 
dc.contributor.authorJimenez, Maribel
dc.identifier.citationBiomed Res Int. 2013;2013:526069.es_ES
dc.description.abstractSand fly salivary proteins are on the spotlight to become vaccine candidates against leishmaniasis and to markers of exposure to sand fly bites due to the host immune responses they elicit. Working with the whole salivary homogenate entails serious drawbacks such as the need for maintaining sand fly colonies and the laborious task of glands dissection. In order to overcome these difficulties, producing recombinant proteins of different vectors has become a major task. In this study, a cDNA library was constructed with the salivary glands of Phlebotomus perniciosus from Madrid, Spain, the most widespread vector of Leishmania infantum in the Mediterranean basin. Analysis of the cDNA sequences showed several polymorphisms among the previously described salivary transcripts. The apyrase SP01B and the D7-related protein SP04 were successfully cloned, expressed in Escherichia coli, and purified. Besides, recombinant proteins were recognized by sera of hamsters and mice previously immunized with saliva through the exposure to uninfected sand fly bites. These results suggest that these two recombinant proteins conserved their immunogenic properties after expression in a prokaryote system. Therefore, this work contributes to expand the knowledge of P. perniciosus saliva that would be eventually used for the development of tools for vector control programs.es_ES
dc.description.sponsorshipThis study was partially funded by the Spanish Ministry of Science & Innovation (Project AGL2008-01592) and by EU grant GOCE-2003-010284 EDENext and is catalogued by the EDENext Steering Committee as EDENext 082 ( The contents of this paper are the sole responsibility of the authors and do not necessarily reflect the views of the European Commission. Inés Martín-Martín is a Ph. D. recipient of a fellowship from the Spanish Ministry of Science & Innovation (FPI-MICINN).es_ES
dc.publisherHindawi es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshApyrase es_ES
dc.subject.meshBase Sequence es_ES
dc.subject.meshCricetinae es_ES
dc.subject.meshGene Library es_ES
dc.subject.meshLeishmania infantum es_ES
dc.subject.meshLeishmaniasis es_ES
dc.subject.meshMice es_ES
dc.subject.meshPhlebotomus es_ES
dc.subject.meshPhylogeny es_ES
dc.subject.meshSaliva es_ES
dc.subject.meshSalivary Proteins and Peptides es_ES
dc.subject.meshSequence Analysis, DNA es_ES
dc.subject.meshSpain es_ES
dc.titleMolecular and immunogenic properties of apyrase SP01B and D7-related SP04 recombinant salivary proteins of Phlebotomus perniciosus from Madrid, Spaines_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.contributor.funderEuropean Union
dc.identifier.journalBioMed research internationales_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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