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dc.contributor.authorPalladino, Claudia
dc.contributor.authorEzeonwumelu, Ifeanyi Jude
dc.contributor.authorMarcelino, Rute
dc.contributor.authorBriz, Veronica 
dc.contributor.authorMoranguinho, Inês
dc.contributor.authorSerejo, Fátima
dc.contributor.authorVelosa, José Fernando
dc.contributor.authorMarinho, Rui Tato
dc.contributor.authorBorrego, Pedro
dc.contributor.authorTaveira, Nuno
dc.date.accessioned2020-04-07T07:41:57Z
dc.date.available2020-04-07T07:41:57Z
dc.date.issued2018
dc.identifier.citationSci Rep. 2018 Aug 16;8(1):12266.es_ES
dc.identifier.issn2045-2322es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9451
dc.description.abstractAny successful strategy to prevent and control HCV infection requires an understanding of the epidemic behaviour among the different genotypes. Here, we performed the first characterization of the epidemic history and transmission dynamics of HCV subtypes in Portugal. Direct sequencing of NS5B was performed on 230 direct-acting antiviral drugs (DAA)-treatment naïve patients in Lisbon. Phylogenetic analysis was used for subtyping and transmission cluster identification. Bayesian methods were used to reconstruct the epidemic history of HCV subtypes. Sequences were analysed for resistance-associated substitutions (RAS). The majority of strains were HCV-GT1 (62.6%), GT3 (18.3%, all subtype 3a) and GT4 (16.1%). Among GT1, the most frequent were subtypes 1a (75.5%) and 1b (24.5%). Polyphyletic patterns were found in all but 12 lineages suggesting multiple introductions of the different subtypes in this population. Five distinct epidemics were identified. The first significant HCV epidemic in Portugal occurred between 1930s and 1960s, was caused almost exclusively by GT1b and was likely associated with blood transfusions. Rapid expansion of GT3a occurred in the 1960s and GT1a in the 1980s, associated with intravenous drug use. The most recent epidemics were caused by GT4a and GT4d and seem to be associated with the resurgence of opioid use. The C316N substitution was found in 31.4% of GT1b-patients. Close surveillance of patients bearing this mutation and undergoing dasabuvir-based regimens will be important to determine its impact on treatment outcome.es_ES
dc.description.sponsorshipThe authors would like to thank all the patients for their participation. C.P. is supported by the Portuguese ‘Fundação para a Ciência e Tecnologia’ (FCT) (grant number SFRH/BPD/77448/2011, part of the EDCTP2 program supported by the European Union). R.M. was supported by a Ph.D. scholarship by the Portuguese ‘Fundação para a Ciência e Tecnologia’ (FCT). V.B. is supported by the Miguel Servet program run by the ‘Fondo de Investigación Sanitaria’ (ISCIII) (grant number CP13/00098). I.M. is supported by the Portuguese ‘Fundação para a Ciência e Tecnologia’ (FCT) (grant number SFRH/BD/131062/2017).es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Group es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAdult es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHepacivirus es_ES
dc.subject.meshHepatitis C, Chronic es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMale es_ES
dc.subject.meshMiddle Aged es_ES
dc.subject.meshMolecular Epidemiology es_ES
dc.subject.meshPolymorphism, Genetic es_ES
dc.subject.meshPortugal es_ES
dc.subject.meshSofosbuvir es_ES
dc.subject.meshSulfonamides es_ES
dc.subject.meshUracil es_ES
dc.subject.meshViral Nonstructural Proteins es_ES
dc.subject.meshGenotype es_ES
dc.titleEpidemic history of hepatitis C virus genotypes and subtypes in Portugales_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID30116054es_ES
dc.format.volume8es_ES
dc.format.number1es_ES
dc.format.page12266es_ES
dc.identifier.doi10.1038/s41598-018-30528-0es_ES
dc.contributor.funderUnión Europea 
dc.contributor.funderFundação para a Ciência e Tecnologia (Portugal) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.description.peerreviewedes_ES
dc.identifier.e-issn2045-2322es_ES
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-018-30528-0es_ES
dc.identifier.journalScientific reportses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/SFRH/BPD/77448/2011es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/SFRH/BD/131062/2017es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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