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dc.contributor.authorTorrellas, Annhymariet
dc.contributor.authorFerrer, Elizabeth
dc.contributor.authorCruz, Israel 
dc.contributor.authorLima, Héctor de
dc.contributor.authorDelgado, Olinda
dc.contributor.authorRangel, José Carrero
dc.contributor.authorBravo, José Arturo
dc.contributor.authorChicharro, Carmen 
dc.contributor.authorLlanes-Acevedo, Ivonne Pamela 
dc.contributor.authorMiles, Michael A
dc.contributor.authorFeliciangeli, María Dora
dc.identifier.citationMem Inst Oswaldo Cruz. 2018 Dec 6;113(12):e180323.es_ES
dc.description.abstractBACKGROUND The transmission routes for American cutaneous leishmaniasis (ACL) are in flux, so studies examining its transmission in humans, mammalian hosts, and sand fly vectors are urgently needed. OBJECTIVES The aim of this work was understand the epidemiological cycles of Leishmania spp., which causes ACL in the Andean Region of Venezuela, by identifying the Leishmania and the sand fly species involved in human and dog infections. METHODS Thirty-one biopsies from patients in Mérida and Táchira states with suspected ACL were studied by both parasitological tests (cultures and hamster inoculation) and a molecular test [Internal transcribed spacer 1 (ITS1) nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)]. We also conducted a survey to detect Leishmania infection in dogs (Immunifluorescence antibody test and ITS1 nested PCR-RFLP) and sand flies (ITS1 nested PCR-RFLP) from El Carrizal, a highly endemic focus of ACL in Venezuela. FINDINGS Three different Leishmania species were identified in the clinical samples from humans (Leishmania braziliensis, L. guyanensis, and L. mexicana) and dogs (L. guyanensis and L. mexicana). The predominant sand fly species found were those from the Verrucarum group (infected with L. mexicana) and Lutzomyia migonei (infected with L. guyanensis and L. mexicana). MAIN CONCLUSIONS We show that Lu. migonei may be the putative vector in two ACL epidemiological cycles, involving L. guyanensis and L. mexicana. We also report for the first time the presence of L. guyanensis in domestic animals.es_ES
dc.description.sponsorshipFinancial support: European Commission project “Control strategies for visceral leishmaniasis (VL) and mucocutaneous leishmaniasis (MCL) in South America: applications of molecular epidemiology” (EC contract INCO-CT2005-015407, LeishEpiNetSA) and by the Ministerio de Ciencia y Tecnología (MPPCTI/FONACIT), Project MISIÓN CIENCIA - Leishmaniasis, 2008000911 - 2. * Present address: Foundation for Innovative New Diagnostics, Geneva, Switzerland. † In memoriames_ES
dc.subject.meshAnimals es_ES
dc.subject.meshDNA, Ribosomal Spacer es_ES
dc.subject.meshDogs es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHumans es_ES
dc.subject.meshInsect Vectors es_ES
dc.subject.meshLeishmania braziliensis es_ES
dc.subject.meshLeishmania guyanensis es_ES
dc.subject.meshLeishmania mexicana es_ES
dc.subject.meshLeishmaniasis, Cutaneous es_ES
dc.subject.meshMolecular Typing es_ES
dc.subject.meshPolymerase Chain Reaction es_ES
dc.subject.meshPolymorphism, Restriction Fragment Length es_ES
dc.subject.meshPsychodidae es_ES
dc.subject.meshVenezuela es_ES
dc.titleMolecular typing reveals the co-existence of two transmission cycles of American cutaneous leishmaniasis in the Andean Region of Venezuela with Lutzomyia migonei as the vectores_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderUnión Europea. Comisión Europea 
dc.contributor.funderMinisterio de Ciencia y Tecnología (España) 
dc.identifier.journalMemorias do Instituto Oswaldo Cruzes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/EC contract INCO-CT2005-015407, LeishEpiNetSA)es_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/Project MISIÓN CIENCIA - Leishmaniasis, 2008000911 - 2es_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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