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dc.contributor.authorPerez-Sautu, Unai 
dc.contributor.authorWiley, Michael Ross
dc.contributor.authorIglesias-Caballero, Maria 
dc.contributor.authorPozo Sanchez, Francisco 
dc.contributor.authorPrieto, Karla
dc.contributor.authorChitty, Joseph Alex
dc.contributor.authorGarcía-García, María Luz
dc.contributor.authorCalvo, Cristina
dc.contributor.authorCasas Flecha, Inmaculada 
dc.contributor.authorPalacios, Gustavo
dc.identifier.citationEmerg Microbes Infect. 2019;8(1):1054-1065.es_ES
dc.description.abstractDespite the advanced PCR-based assays available, a fraction of the pediatric respiratory infections remain unexplained every epidemic season, and there is a perception that novel viruses might be present in these specimens. We systematically collected samples from a prospective cohort of pediatric patients with respiratory infections, that returned negative results by validated molecular RT-PCR assays, and studied them with a target-independent, high-throughput sequencing-based approach. We also included a matched cohort of children with no symptoms of respiratory infection, as a contrast study population. More than fifty percent of the specimens from the group of patients with unexplained respiratory infections were resolved. However, the higher rate of detection was not due to the presence of novel viruses, but to the identification of well-known viral respiratory pathogens. Our results show that already known viral pathogens are responsible for the majority of cases that remain unexplained after the epidemic season. High-throughput sequencing approaches that use pathogen-specific probes are easier to standardize because they ensure reproducible library enrichment and sequencing. In consequence, these techniques might be desirable from a regulatory standpoint for diagnostic laboratories seeking to benefit from the many advantages of these sequencing technologies.es_ES
dc.description.sponsorshipThis work was supported by The Joint Science and Technology Office for Chemical and Biological Defense (JSTO-CBD) of the Defense Threat Reduction Agency (DTRA) under plan #CB10246. This study has been partially supported by Fondo de Investigaciones Sanitarias (FIS) – Spanish Health Research Fund Grant PI12/01291, and Instituto de Salud Carlos III (ISCIII), Grant PI15CIII/00028.es_ES
dc.publisherTaylor & Francis es_ES
dc.subjectPediatric respiratory infectiones_ES
dc.subjectHigh-throughput sequencinges_ES
dc.subjectRespiratory viruseses_ES
dc.subjectViral genomicses_ES
dc.subject.meshAdolescent es_ES
dc.subject.meshChild es_ES
dc.subject.meshChild, Preschool es_ES
dc.subject.meshFemale es_ES
dc.subject.meshGenome, Viral es_ES
dc.subject.meshHigh-Throughput Nucleotide Sequencing es_ES
dc.subject.meshHumans es_ES
dc.subject.meshInfant es_ES
dc.subject.meshInfant, Newborn es_ES
dc.subject.meshMale es_ES
dc.subject.meshProspective Studies es_ES
dc.subject.meshRespiratory Tract Infections es_ES
dc.subject.meshVirus Diseases es_ES
dc.subject.meshViruses es_ES
dc.titleTarget-independent high-throughput sequencing methods provide evidence that already known human viral pathogens play a main role in respiratory infections with unexplained etiologyes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderDefense Threat Reduction Agency (Estados Unidos) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.identifier.journalEmerging microbes & infectionses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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