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dc.contributor.authorSevilla, Elena 
dc.contributor.authorGonzalez, Luis Miguel 
dc.contributor.authorLuque, Daniel 
dc.contributor.authorGray, Jeremy
dc.contributor.authorMontero-Clemente, Estrella
dc.identifier.citationSci Rep. 2018 Sep 20;8(1):14116.es_ES
dc.description.abstractBased on confocal fluorescence and bright field video microscopy, we present detailed observations on the processes of invasion and egress of erythrocytes by the apicomplexan parasite Babesia divergens. Time-lapse images reveal numerous unexpected findings associated with the dynamics of B. divergens and its ability to manipulate the erythrocyte during both processes in its asexual cycle under in vitro conditions. Despite the speed at which these processes occur and the small size of the parasite, we capture infective merozoites moving vigorously and causing striking deformations in the erythrocyte's plasma membrane during an active invasion. We also observed intraerythrocytic dynamic stages as paired pyriforms, double paired pyriforms, tetrads, unattached pyriform sister cells and multiple parasite stages resulting in the release of large numbers of merozoites over a short period. Of considerable interest is that time-lapse images reveal a novel mechanism of egress used by B. divergens to exit the human erythrocyte. The release occurs when B. divergens parasites establish contacts with the plasma membrane of the erythrocyte from within, before exiting the cell. Visualization and analysis of the images enabled us to obtain useful information and broaden our knowledge of complex and crucial events involved with parasitisation of human erythrocytes by B. divergens.es_ES
dc.description.sponsorshipThis work was funded by grants from Ministerio de Economía y Competitividad from Spain (AGL2010-21774, AGL2014-56193 R to EM and LMG). E. Sevilla was awarded a research fellowship from Plan Estatal de Investigación Científica y Técnica y de Innovación, Ministerio de Economía y Competitividad, Spain.es_ES
dc.publisherNature Publishing Group es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshBabesia es_ES
dc.subject.meshBabesiosis es_ES
dc.subject.meshCattle es_ES
dc.subject.meshCattle Diseases es_ES
dc.subject.meshErythrocyte Membrane es_ES
dc.subject.meshErythrocytes es_ES
dc.subject.meshTime-Lapse Imaging es_ES
dc.titleKinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopyes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.identifier.journalScientific reportses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/AGL2014-56193 Res_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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