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dc.contributor.authorGarcia-Rodas, Rocio 
dc.contributor.authorTrevijano-Contador, Nuria 
dc.contributor.authorRomán, Elvira
dc.contributor.authorJanbon, Guilhem
dc.contributor.authorMoyrand, Frédérique
dc.contributor.authorPla, Jesús
dc.contributor.authorCasadevall, Arturo
dc.contributor.authorZaragoza, Oscar 
dc.date.accessioned2020-02-21T10:58:09Z
dc.date.available2020-02-21T10:58:09Z
dc.date.issued2015
dc.identifier.citationFront Microbiol. 2015 Aug 12;6:798.es_ES
dc.identifier.issn1664-302Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9132
dc.description.abstractCryptococcus neoformans is an opportunistic fungal pathogen that has several well-described virulence determinants. A polysaccharide capsule and the ability to produce melanin are among the most important. Melanization occurs both in vitro, in the presence of catecholamine and indole compounds, and in vivo during the infection. Despite the importance of melanin production for cryptococcal virulence, the component and mechanisms involved in its synthesis have not been fully elucidated. In this work, we describe the role of a G1/S cyclin (Cln1) in the melanization process. Cln1 has evolved specifically with proteins present only in other basidiomycetes. We found that Cln1 is required for the cell wall stability and production of melanin in C. neoformans. Absence of melanization correlated with a defect in the expression of the LAC1 gene. The relation between cell cycle elements and melanization was confirmed by the effect of drugs that cause cell cycle arrest at a specific phase, such as rapamycin. The cln1 mutant was consistently more susceptible to oxidative damage in a medium that induces melanization. Our results strongly suggest a novel and hitherto unrecognized role for C. neoformans Cln1 in the expression of virulence traits.es_ES
dc.description.sponsorshipWe thank Rajendra Uphadya (Washington University School of Medicine, St. Louis, MI, USA) for providing the sequence of oligonucleotides for 18s gene used in this article. RG-R was supported by a FPI fellowship (reference BES-2009-015913) from the Spanish Ministry of Economics and Competitivity. NT-C is supported by a FPI fellowship (reference BES-2012-051837). OZ is funded by grant SAF2011-25140 and SAF2014-54336 from the Spanish Ministry for Economics and Competitivityes_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCryptococcus CLN1es_ES
dc.subjectcell walles_ES
dc.subjectlaccasees_ES
dc.subjectmelanines_ES
dc.titleRole of Cln1 during melanization of Cryptococcus neoformanses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID26322026es_ES
dc.format.volume6es_ES
dc.format.page798es_ES
dc.identifier.doi10.3389/fmicb.2015.00798es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2015.00798es_ES
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BES-2009-015913es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BES-2012-051837es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2011-25140es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2014-54336es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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