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dc.contributor.authorMohedano Macias, Laura 
dc.contributor.authorAmblar, Monica 
dc.contributor.authorde la Fuente, Alicia
dc.contributor.authorWells, Jerry M
dc.contributor.authorLópez, Paloma
dc.identifier.citationFront Microbiol. 2016 Aug 25;7:1326.es_ES
dc.description.abstractThe YycFG (also known as WalRK, VicRK, MicAB, or TCS02) two-component system (TCS) is highly conserved among Gram-positive bacteria with a low G+C content. In Streptococcus pneumoniae the YycF response regulator has been reported to be essential due to its control of pcsB gene expression. Previously we showed that overexpression of yycF in S. pneumoniae TIGR4 altered the transcription of genes involved in cell wall metabolism and fatty acid biosynthesis, giving rise to anomalous cell division and increased chain length of membrane fatty acids. Here, we have overexpressed the yycFG system in TIGR4 wild-type strain and yycF in a TIGR4 mutant depleted of YycG, and analyzed their effects on expression of proteins involved in fatty acid biosynthesis during activation of the TCS. We demonstrate that transcription of the fab genes and levels of their products were only altered in the YycF overexpressing strain, indicating that the unphosphorylated form of YycF is involved in the regulation of fatty acid biosynthesis. In addition, DNA-binding assays and in vitro transcription experiments with purified YycF and the promoter region of the FabTH-acp operon support a direct inhibition of transcription of the FabT repressor by YycF, thus confirming the role of the unphosphorylated form in transcriptional regulation.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Industry and Laboratorios SALVAT within the European project Eureka Σ! 3554-DEADBUGS by the Spanish Ministry of Economics and Competitiveness grants AGL2012-40084C03-01 and AGL2015-65010-C3-1-R, and by the European Union grant FP7-PEOPLE-ITN-2008-238490. The work at the CIB was performed under the auspices of the Consejo Superior de Investigaciones Científicas.es_ES
dc.publisherFrontiers Media es_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.subjectEssential response regulatores_ES
dc.subjectFatty acid biosynthesises_ES
dc.subjectTranscriptional regulationes_ES
dc.subjectTwo component systemses_ES
dc.titleThe Response Regulator YycF Inhibits Expression of the Fatty Acid Biosynthesis Repressor FabT in Streptococcus pneumoniaees_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderUnión Europea 
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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