Show simple item record

dc.contributor.authorMartin-Vicente, Maria 
dc.contributor.authorResino, Salvador 
dc.contributor.authorMartinez, Isidoro 
dc.date.accessioned2020-02-10T12:02:06Z
dc.date.available2020-02-10T12:02:06Z
dc.date.issued2019
dc.identifier.citationBiomolecules. 2019 Apr 28;9(5). pii: E165.es_ES
dc.identifier.issn2218-273Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9069
dc.description.abstractHuman respiratory syncytial virus (HRSV) infection is a common cause of severe lower respiratory tract diseases such as bronchiolitis and pneumonia. Both virus replication and the associated inflammatory immune response are believed to be behind these pathologies. So far, no vaccine or effective treatment is available for this viral infection. With the aim of finding new strategies to counteract HRSV replication and modulate the immune response, specific small interfering RNAs (siRNAs) were generated targeting the mRNA coding for the viral fusion (F) protein or nucleoprotein (N), or for two proteins involved in intracellular immune signaling, which are named tripartite motif-containing protein 25 (TRIM25) and retinoic acid-inducible gene-I (RIG-I). Furthermore, two additional bispecific siRNAs were designed that silenced F and TRIM25 (TRIM25/HRSV-F) or N and RIG-I (RIG-I/HRSV-N) simultaneously. All siRNAs targeting N or F, but not those silencing TRIM25 or RIG-I alone, significantly reduced viral titers. However, while siRNAs targeting F inhibited only the expression of the F mRNA and protein, the siRNAs targeting N led to a general inhibition of viral mRNA and protein expression. The N-targeting siRNAs also induced a drastic decrease in the expression of genes of the innate immune response. These results show that both virus replication and the early innate immune response can be regulated by targeting distinct viral products with siRNAs, which may be related to the different role of each protein in the life cycle of the virus.es_ES
dc.description.sponsorshipThis research was funded by the Instituto de Salud Carlos III, gran number PI15CIII/0024 to IM.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectInnate immune responsees_ES
dc.subjectRespiratory syncytial viruses_ES
dc.subjectsiRNAes_ES
dc.subjectTreatmentes_ES
dc.titlesiRNA-Mediated Simultaneous Regulation of the Cellular Innate Immune Response and Human Respiratory Syncytial Virus Replicationes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID31035368es_ES
dc.format.volume9es_ES
dc.format.number5es_ES
dc.format.page165es_ES
dc.identifier.doi10.3390/biom9050165es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.description.peerreviewedes_ES
dc.identifier.e-issn2218-273Xes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/biom9050165es_ES
dc.identifier.journalBiomoleculeses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI15CIII/0024es_ES
dc.rights.accessRightsopen accesses_ES


Files in this item

Acceso Abierto
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Atribución 4.0 Internacional
This item is licensed under a: Atribución 4.0 Internacional