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dc.contributor.author | Van der Auwera, Gert | |
dc.contributor.author | Bart, Aldert | |
dc.contributor.author | Chicharro, Carmen | |
dc.contributor.author | Cortes, Sofia | |
dc.contributor.author | Davidsson, Leigh | |
dc.contributor.author | Di Muccio, Trentina | |
dc.contributor.author | Dujardin, Jean-Claude | |
dc.contributor.author | Felger, Ingrid | |
dc.contributor.author | Paglia, Maria Grazia | |
dc.contributor.author | Grimm, Felix | |
dc.contributor.author | Harms, Gundel | |
dc.contributor.author | Jaffe, Charles L | |
dc.contributor.author | Manser, Monika | |
dc.contributor.author | Ravel, Christophe | |
dc.contributor.author | Robert-Gangneux, Florence | |
dc.contributor.author | Roelfsema, Jeroen | |
dc.contributor.author | Töz, Seray | |
dc.contributor.author | Verweij, Jaco J | |
dc.contributor.author | Chiodini, Peter L | |
dc.date.accessioned | 2020-02-03T08:51:54Z | |
dc.date.available | 2020-02-03T08:51:54Z | |
dc.date.issued | 2016-12-08 | |
dc.identifier.citation | Euro Surveill. 2016 Dec 8;21(49). pii: 30418. | es_ES |
dc.identifier.issn | 1025-496X | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/8997 | |
dc.description.abstract | Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species. Sixteen clinical laboratories in 10 European countries, plus Israel and Turkey, conducted a study to assess their genotyping performance. DNA from 21 promastigote cultures of 13 species was analysed blindly by the routinely used typing method. Five different molecular targets were used, which were analysed with PCR-based methods. Different levels of identification were achieved, and either the Leishmania subgenus, species complex, or actual species were reported. The overall error rate of strains placed in the wrong complex or species was 8.5%. Various reasons for incorrect typing were identified. The study shows there is considerable room for improvement and standardisation of Leishmania typing. The use of well validated standard operating procedures is recommended, covering testing, interpretation, and reporting guidelines. Application of the internal transcribed spacer 1 of the rDNA array should be restricted to Old World samples, while the heat-shock protein 70 gene and the mini-exon can be applied globally. | es_ES |
dc.description.sponsorship | The authors would like to thank the ESCMID study group for Clinical Parasitology (ESGCP, headed by Titia Kortbeek, National Institute for Public Health and the Environment, RIVM, Bilthoven, The Netherlands) for financial support (ESCMID Study Group Research Grant 2013 to Peter L. Chiodini). We are grateful to Jean-Pierre Gangneux (ESGCP coordinator for leishmaniasis, Centre Hospitalier Universitaire de Rennes, Rennes, France) and the LeishMan consortium [www.leishman.eu] for conceptual support and promoting the study. We acknowledge the valuable comments of Titia Kortbeek, and the technical assistance of Sofia Andersson (The Public Health Agency of Sweden, Stockholm, Sweden), José M. Cristóvão (Global Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, UNL, Lisbon, Portugal), Mehmet Karakus (Ege University, Faculty of Medicine, Department of Parasitology, Izmir, Turkey), Ilse Maes (Institute of Tropical Medicine, Antwerp, Belgium), Abed Nasereddin (Hebrew University, Hadassah Medical Centre, Jerusalem, Israel), Chris Stalder (Swiss Tropical and Public Health Institute, Basel, Switzerland), Antonietta Toffoletti and Antonella Vulcano (National Institute for Infectious Diseases (INMI) Lazzaro Spallanzani, Rome, Italy), Carla Wassenaar (Academic Medical Center, Amsterdam, The Netherlands), Julie Watson and Spencer Polley (Hospital for Tropical Diseases, London, United Kingdom). Gert Van der Auwera is supported by the Third Framework program between ITM and the Belgian Directorate General for Development. Peter L. Chiodini is supported by the Biomedical Research Centre of the University College London Hospitals and National Institute for Health Research. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | European Centre for Disease Prevention and Control (ECDC) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Typing | es_ES |
dc.subject | hsp70 | es_ES |
dc.subject | Kinetoplast DNA | es_ES |
dc.subject | Leishmaniasis | es_ES |
dc.subject | Mini-exon | es_ES |
dc.subject | rDNA ITS1 | es_ES |
dc.subject.mesh | DNA, Kinetoplast | es_ES |
dc.subject.mesh | DNA, Protozoan | es_ES |
dc.subject.mesh | DNA, Ribosomal | es_ES |
dc.subject.mesh | Europe | es_ES |
dc.subject.mesh | Genotype | es_ES |
dc.subject.mesh | HSP70 Heat-Shock Proteins | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Israel | es_ES |
dc.subject.mesh | Laboratories | es_ES |
dc.subject.mesh | Leishmania | es_ES |
dc.subject.mesh | Leishmaniasis | es_ES |
dc.subject.mesh | Polymerase Chain Reaction | es_ES |
dc.subject.mesh | Polymorphism, Restriction Fragment Length | es_ES |
dc.subject.mesh | Sensitivity and Specificity | es_ES |
dc.subject.mesh | Turkey | es_ES |
dc.title | Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014 | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 27983510 | es_ES |
dc.format.volume | 21 | es_ES |
dc.format.number | 49 | es_ES |
dc.identifier.doi | 10.2807/1560-7917.ES.2016.21.49.30418 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1560-7917 | es_ES |
dc.relation.publisherversion | https://doi.org/10.2807/1560-7917.ES.2016.21.49.30418 | es_ES |
dc.identifier.journal | Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |