Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/8892
MicroRNA Profile of HCV Spontaneous Clarified Individuals, Denotes Previous HCV Infection
Brochado-Kith, Oscar ISCIII | Gomez-Sanz, Alicia ISCIII | Real, Luis Miguel | Crespo García, Javier | Ryan Murúa, Pablo | Macías, Juan | Cabezas González, Joaquín | Troya, Jesús | Pineda, Juan Antonio | Arias Loste, María Teresa | Díez Viñas, Victorino | Jimenez-Sousa, Maria Angeles ISCIII | Medrano, Luz Maria ISCIII | Cuesta de la Plaza, Isabel ISCIII | Monzon-Fernandez, Sara ISCIII | Resino, Salvador ISCIII | Fernandez-Rodriguez, Amanda ISCIII
J Clin Med. 2019 Jun 14;8(6). pii: E849.
Factors involved in the spontaneous cleareance of a hepatitis C (HCV) infection are related to both HCV and the interaction with the host immune system, but little is known about the consequences after a spontaneous resolution. The main HCV extrahepatic reservoir is the peripheral blood mononuclear cells (PBMCs), and their transcriptional profile provides us information of innate and adaptive immune responses against an HCV infection. MicroRNAs regulate the innate and adaptive immune responses, and they are actively involved in the HCV cycle. High Throughput sequencing was used to analyze the miRNA profiles from PBMCs of HCV chronic naïve patients (CHC), individuals that spontaneously clarified HCV (SC), and healthy controls (HC). We did not find any differentially expressed miRNAs between SC and CHC. However, both groups showed similar expression differences (21 miRNAs) with respect to HC. This miRNA signature correctly classifies HCV-exposed (CHC and SC) vs. HC, with the has-miR-21-3p showing the best performance. The potentially targeted molecular pathways by these 21 miRNAs mainly belong to fatty acids pathways, although hippo signaling, extracellular matrix (ECM) interaction, proteoglycans-related, and steroid biosynthesis pathways were also altered. These miRNAs target host genes involved in an HCV infection. Thus, an HCV infection promotes molecular alterations in PBMCs that can be detected after an HCV spontaneous resolution, and the 21-miRNA signature is able to identify HCV-exposed patients (either CHC or SC).
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