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dc.contributor.author | Gil, Horacio | |
dc.contributor.author | Fernandez-Garcia, Aurora | |
dc.contributor.author | Mosquera Gutierrez, Maria del Mar | |
dc.contributor.author | Hübschen, Judith M | |
dc.contributor.author | Castellanos-Nadal, Ana Maria | |
dc.contributor.author | De Ory, Fernando de | |
dc.contributor.author | Masa-Calles, Josefa | |
dc.contributor.author | Echevarria, Juan Emilio | |
dc.date.accessioned | 2019-11-28T13:04:11Z | |
dc.date.available | 2019-11-28T13:04:11Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | PLoS One. 2018 Jul 16;13(7):e0199975. | es_ES |
dc.identifier.issn | 1932-6203 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/8719 | |
dc.description.abstract | In recent decades, vaccination has substantially reduced the number of measles cases to levels close to the elimination stage. However, major measles outbreaks occurred in Europe during 2010-2012, after the introduction of the D4-Enfield lineage. We have performed a molecular characterization of 75 measles virus genotype D4 strains from patients infected in Spain between 2004 and 2012 by sequencing the N-450 region and the M-F non-coding region (M-F NCR) in order to identify genetic features of these viruses. The analysis of the N-450 region confirmed that all samples obtained since 2008 belonged to variants or sets of identical sequences of the D4-Enfield lineage, including a new one named MVs/Madrid.ESP/46.10/. Analysis of the M-F NCR showed insertions and deletions associated with previously described, uncommon non-standard genome length measles viruses. This genetic feature was identified in the D4-Enfield lineage viruses, but not in the other D4 viruses that were circulating in Spain before 2008, suggesting that these non-standard length M-F NCR sequences are characteristic of the D4-Enfield lineage. The results of the phylogenetic analysis of Spanish M-F NCRs suggest higher resolution in discriminating strains than did the N-450 analysis. In addition, the results of the analysis of the M-F NCR on the MVs/Madrid.ESP/46.10/ sub-lineage seem to support the potential utility of this region as a tool for epidemiological surveillance complementary to the N-450 region, as previously suggested. Further investigation on this question, as well as the surveillance of new potentially emerging strains with non-standard length M-F NCR are strongly recommended as part of future strategies for measles elimination. | es_ES |
dc.description.sponsorship | This work was supported by the Fondo de Investigación Sanitaria (FIS PI12/02006) y el Instituto de Salud Carlos III (PI15CIII/00023). AFG was funded by CIBER de Epidemiología y Salud Pública (ESPG13). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (PLOS) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Measles | es_ES |
dc.subject.mesh | Measles virus | es_ES |
dc.subject.mesh | Phylogeny | es_ES |
dc.subject.mesh | Spain | es_ES |
dc.subject.mesh | Disease Outbreaks | es_ES |
dc.subject.mesh | Genotype | es_ES |
dc.title | Measles virus genotype D4 strains with non-standard length M-F non-coding region circulated during the major outbreaks of 2011-2012 in Spain | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 30011283 | es_ES |
dc.format.volume | 13 | es_ES |
dc.format.number | 7 | es_ES |
dc.format.page | e0199975 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0199975 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1932-6203 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0199975 | es_ES |
dc.identifier.journal | PloS one | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Epidemiología | |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/FIS PI12/02006 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PI15CIII/00023 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/ESPG13 | es_ES |
dc.rights.accessRights | open access | es_ES |