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dc.contributor.authorGil, Horacio 
dc.contributor.authorFernández-García, Aurora 
dc.contributor.authorMosquera, Maria del Mar 
dc.contributor.authorHübschen, Judith M
dc.contributor.authorCastellanos-Nadal, Ana Maria 
dc.contributor.authorOry, Fernando de 
dc.contributor.authorMasa-Calles, Josefa 
dc.contributor.authorEchevarria, Juan Emilio
dc.identifier.citationPLoS One. 2018 Jul 16;13(7):e0199975.es_ES
dc.description.abstractIn recent decades, vaccination has substantially reduced the number of measles cases to levels close to the elimination stage. However, major measles outbreaks occurred in Europe during 2010-2012, after the introduction of the D4-Enfield lineage. We have performed a molecular characterization of 75 measles virus genotype D4 strains from patients infected in Spain between 2004 and 2012 by sequencing the N-450 region and the M-F non-coding region (M-F NCR) in order to identify genetic features of these viruses. The analysis of the N-450 region confirmed that all samples obtained since 2008 belonged to variants or sets of identical sequences of the D4-Enfield lineage, including a new one named MVs/Madrid.ESP/46.10/. Analysis of the M-F NCR showed insertions and deletions associated with previously described, uncommon non-standard genome length measles viruses. This genetic feature was identified in the D4-Enfield lineage viruses, but not in the other D4 viruses that were circulating in Spain before 2008, suggesting that these non-standard length M-F NCR sequences are characteristic of the D4-Enfield lineage. The results of the phylogenetic analysis of Spanish M-F NCRs suggest higher resolution in discriminating strains than did the N-450 analysis. In addition, the results of the analysis of the M-F NCR on the MVs/Madrid.ESP/46.10/ sub-lineage seem to support the potential utility of this region as a tool for epidemiological surveillance complementary to the N-450 region, as previously suggested. Further investigation on this question, as well as the surveillance of new potentially emerging strains with non-standard length M-F NCR are strongly recommended as part of future strategies for measles elimination.es_ES
dc.description.sponsorshipThis work was supported by the Fondo de Investigación Sanitaria (FIS PI12/02006) y el Instituto de Salud Carlos III (PI15CIII/00023). AFG was funded by CIBER de Epidemiología y Salud Pública (ESPG13). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshHumans es_ES
dc.subject.meshMeasles es_ES
dc.subject.meshMeasles virus es_ES
dc.subject.meshPhylogeny es_ES
dc.subject.meshSpain es_ES
dc.subject.meshDisease Outbreaks es_ES
dc.subject.meshGenotype es_ES
dc.titleMeasles virus genotype D4 strains with non-standard length M-F non-coding region circulated during the major outbreaks of 2011-2012 in Spaines_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.centroISCIII::Centro Nacional de Epidemologíaes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/FIS PI12/02006es_ES

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