Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/8710
Título
Identification of new and unusual rev and nef transcripts expressed by an HIV type 1 primary isolate
Autor(es)
Vega Rocha, Yolanda ISCIII | Delgado, Elena ISCIII | Carrera, Cristina ISCIII | Nebreda, Paloma ISCIII | Fernandez-Garcia, Aurora ISCIII | Cuevas, Maria Teresa ISCIII | Perez-Alvarez, Lucia ISCIII | Thomson, Michael M ISCIII
Fecha de publicación
2013-07
Cita
AIDS Res Hum Retroviruses. 2013 Jul;29(7):1075-8. doi: 10.1089/AID.2013.0053. Epub 2013 May 8.
Idioma
Inglés
Tipo de documento
journal article
Resumen
We analyzed RNA splice site usage in three HIV-1 subtype B primary isolates through reverse transcriptase polymerase chain reaction (RT-PCR) amplification of spliced RNAs using a fluorescently labeled primer, with computerized size determination and quantification of PCR products, which were also identified by clone sequencing. In one isolate, P2149-3, unusual and unreported spliced transcripts were detected. This isolate preferentially used for rev RNA generation a 3' splice site (3'ss) located five nucleotides upstream of A4a, previously identified only in a T cell line-adapted virus and in a group O isolate, and designated A4d. P2149-3 also used an unreported 3'ss for rev RNA generation, designated A4h, located 20 nucleotides upstream of 3'ss A4c. Additionally, unusual nef RNAs using 3'ss A5a and A7a and with exon composition 1.3.7 were identified. The identification of several unusual and unreported spliced transcripts in an HIV-1 primary isolate suggests a greater diversity of splice site usage in HIV-1 than previously appreciated.
MESH
Base Sequence | Genetic Variation | HIV-1 | Humans | Leukocytes, Mononuclear | Molecular Sequence Data | RNA Splice Sites | RNA, Viral | Genes, nef | Genes, rev
Versión en línea
DOI
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