Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/8594
Título
Reactive Oxygen Species Contribute to the Bactericidal Effects of the Fluoroquinolone Moxifloxacin in Streptococcus pneumoniae
Autor(es)
Ferrandiz-Avellano, Maria-Jose ISCIII | Martin-Galiano, Antonio Javier ISCIII | Arnanz, Cristina ISCIII | Zimmerman, T | de la Campa, Adela G ISCIII
Fecha de publicación
2016
Cita
Antimicrob Agents Chemother. 2016;60(1):409-17.
Idioma
Inglés
Tipo de documento
research article
Resumen
We studied the transcriptomic response of Streptococcus pneumoniae to the fluoroquinolone moxifloxacin at a concentration that inhibits DNA gyrase. Treatment of the wild-type strain R6, at a concentration of 10× the MIC, triggered a response involving 132 genes after 30 min of treatment. Genes from several metabolic pathways involved in the production of pyruvate were upregulated. These included 3 glycolytic enzymes, which ultimately convert fructose 6-phosphate to pyruvate, and 2 enzymes that funnel phosphate sugars into the glycolytic pathway. In addition, acetyl coenzyme A (acetyl-CoA) carboxylase was downregulated, likely leading to an increase in acetyl-CoA. When coupled with an upregulation in formate acetyltransferase, an increase in acetyl-CoA would raise the production of pyruvate. Since pyruvate is converted by pyruvate oxidase (SpxB) into hydrogen peroxide (H2O2), an increase in pyruvate would augment intracellular H2O2. Here, we confirm a 21-fold increase in the production of H2O2 and a 55-fold increase in the amount of hydroxyl radical in cultures treated during 4 h with moxifloxacin. This increase in hydroxyl radical through the Fenton reaction would damage DNA, lipids, and proteins. These reactive oxygen species contributed to the lethality of the drug, a conclusion supported by the observed protective effects of an SpxB deletion. These results support the model whereby fluoroquinolones cause redox alterations. The transcriptional response of S. pneumoniae to moxifloxacin is compared with the response to levofloxacin, an inhibitor of topoisomerase IV. Levofloxacin triggers the transcriptional activation of iron transport genes and also enhances the Fenton reaction.
MESH
Acetyl-CoA Carboxylase | Acetyltransferases | Anti-Bacterial Agents | Bacterial Proteins | DNA Topoisomerase IV | Drug Resistance, Multiple, Bacterial | Fluoroquinolones | Fructosephosphates | Gene Deletion | Gene Expression Profiling | Gene Ontology | Glycolysis | Hydrogen Peroxide | Iron | Levofloxacin | Microbial Sensitivity Tests | Molecular Sequence Annotation | Moxifloxacin | Oxidative Stress | Pyruvate Oxidase | Pyruvic Acid | Streptococcus pneumoniae | Transcription, Genetic | Gene Expression Regulation, Bacterial
Versión en línea
DOI
Aparece en las colecciones