Mostrar el registro sencillo del ítem
dc.contributor.author | Domenech, Mirian | |
dc.contributor.author | Ramos-Sevillano, Elisa | |
dc.contributor.author | García, Ernesto | |
dc.contributor.author | Moscoso, Miriam | |
dc.contributor.author | Yuste, Jose Enrique | |
dc.date.accessioned | 2019-11-14T11:09:58Z | |
dc.date.available | 2019-11-14T11:09:58Z | |
dc.date.issued | 2013-07 | |
dc.identifier.citation | Infect Immun. 2013 Jul;81(7):2606-15. doi: 10.1128/IAI.00491-13. Epub 2013 May 6. | es_ES |
dc.identifier.issn | 0019-9567 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/8591 | |
dc.description.abstract | Streptococcus pneumoniae is a frequent member of the microbiota of the human nasopharynx. Colonization of the nasopharyngeal tract is a first and necessary step in the infectious process and often involves the formation of sessile microbial communities by this human pathogen. The ability to grow and persist as biofilms is an advantage for many microorganisms, because biofilm-grown bacteria show reduced susceptibility to antimicrobial agents and hinder recognition by the immune system. The extent of host protection against biofilm-related pneumococcal disease has not been determined yet. Using pneumococcal strains growing as planktonic cultures or as biofilms, we have investigated the recognition of S. pneumoniae by the complement system and its interactions with human neutrophils. Deposition of C3b, the key complement component, was impaired on S. pneumoniae biofilms. In addition, binding of C-reactive protein and the complement component C1q to the pneumococcal surface was reduced in biofilm bacteria, demonstrating that pneumococcal biofilms avoid the activation of the classical complement pathway. In addition, recruitment of factor H, the downregulator of the alternative pathway, was enhanced by S. pneumoniae growing as biofilms. Our results also show that biofilm formation diverts the alternative complement pathway activation by a PspC-mediated mechanism. Furthermore, phagocytosis of pneumococcal biofilms was also impaired. The present study confirms that biofilm formation in S. pneumoniae is an efficient means of evading both the classical and the PspC-dependent alternative complement pathways the host immune system. | es_ES |
dc.description.sponsorship | This work was supported by grants SAF2009-10824; and SAF2012-39444-C02-01/02 from Dirección General de Investigación Científica y Técnica and MINECO, respectively. Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES) is an initiative of the ISCIII. M.D. and E.R-S. were supported by FPI and FPU fellowships, respectively, from Ministerio de Economía y Competitividad. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Microbiology (ASM) | es_ES |
dc.relation | info:eu-repo/grantAgreement/ES/SAF2009-10824 | es_ES |
dc.relation | info:eu-repo/grantAgreement/ES/SAF2012-39444-C02-01/02 | es_ES |
dc.type.hasVersion | AM | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject.mesh | Adult | es_ES |
dc.subject.mesh | Bacterial Proteins | es_ES |
dc.subject.mesh | Complement C3b | es_ES |
dc.subject.mesh | Complement C4b-Binding Protein | es_ES |
dc.subject.mesh | Complement Pathway, Alternative | es_ES |
dc.subject.mesh | Host-Pathogen Interactions | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Microscopy, Confocal | es_ES |
dc.subject.mesh | Neutrophils | es_ES |
dc.subject.mesh | Pneumococcal Infections | es_ES |
dc.subject.mesh | Streptococcus pneumoniae | es_ES |
dc.subject.mesh | Biofilms | es_ES |
dc.subject.mesh | Complement Pathway, Classical | es_ES |
dc.subject.mesh | Immune Evasion | es_ES |
dc.subject.mesh | Phagocytosis | es_ES |
dc.title | Biofilm formation avoids complement immunity and phagocytosis of Streptococcus pneumoniae | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 23649097 | es_ES |
dc.format.volume | 81 | es_ES |
dc.format.number | 7 | es_ES |
dc.format.page | 2606-15 | es_ES |
dc.identifier.doi | 10.1128/IAI.00491-13 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Centro de Investigación Biomedica en Red - CIBER | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1098-5522 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1128/IAI.00491-13 | es_ES |
dc.identifier.journal | Infection and immunity | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |