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dc.contributor.authorAndres-Delgado, Laura 
dc.contributor.authorErnst, Alexander
dc.contributor.authorGalardi-Castilla, Maria 
dc.contributor.authorBazaga, David 
dc.contributor.authorPeralta, Marina 
dc.contributor.authorMunch, Juliane 
dc.contributor.authorGonzalez-Rosa, Juan Manuel 
dc.contributor.authorMarques, Inês
dc.contributor.authorTessadori, Federico
dc.contributor.authorde la Pompa, Jose Luis 
dc.contributor.authorVermot, Julien
dc.contributor.authorMercader, Nadia 
dc.date.accessioned2019-09-11T08:07:26Z
dc.date.available2019-09-11T08:07:26Z
dc.date.issued2019-07
dc.identifier.citationDevelopment. 2019; 146(13):dev174961es_ES
dc.identifier.issn0950-1991es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8335
dc.description.abstractThe epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using a zebrafish model and a combination of genetic tools, pharmacological agents and quantitative in vivo imaging, we reveal that a coordinated collective movement of DP cells drives PE formation. We found that Bmp signaling and the actomyosin cytoskeleton promote constriction of the DP, which enables PE cells to extrude apically. We provide evidence that cell extrusion, which has been described in the elimination of unfit cells from epithelia and the emergence of hematopoietic stem cells, is also a mechanism for PE cells to exit an organized mesothelium and fulfil their developmental fate to form a new tissue layer, the epicardium.es_ES
dc.description.sponsorshipN.M. was funded by the Spanish Ministry of Economy and Competitiveness through grant BFU2014–56970–P (Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016. Programa Estatal de I+D+i Orientada a los Retos de la Sociedad Retos Investigación: Proyectos I+D+i 2016, del Ministerio de Economía competitividad e Industria), and co-funding by Fondo Europeo de Desarrollo Regional (FEDER). N.M. is also supported by the Swiss National Science Foundation grant ANR-SNF 310030E-164245 and the ERC starting grant 337703. L.A-D was funded through the postdoctoral fellowship Ayudas Postdoctorales 2013-2016121212. JL de la Pompa was supported by grants SAF2016-78370-R, CB16/11/00399 (CIBER CV) and RD16/0011/0021 (TERCEL) from the Spanish Ministry of Economy, Industry and Competitiveness (MEIC). J. Vermot is supported by ANR-15-CE13-0015 – liveheart and M. Peralta by a 708312 - ZEBRA-VALVE EU project. The CNIC is supported by the Ministry of Economy, Industry and Competitiveness (MEIC) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (MEIC award SEV-2015-0505).es_ES
dc.language.isoenges_ES
dc.publisherThe Company of Biologists es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectActomyosines_ES
dc.subjectBmpes_ES
dc.subjectCell extrusiones_ES
dc.subjectHeart developmentes_ES
dc.subjectProepicardiumes_ES
dc.subjectZebrafishes_ES
dc.titleActin dynamics and the Bmp pathway drive apical extrusion of proepicardial cellses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID31175121es_ES
dc.format.volume146es_ES
dc.format.number13es_ES
dc.format.pagedev174961es_ES
dc.identifier.doi10.1242/dev.174961es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España)
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)
dc.contributor.funderSwiss National Science Foundation
dc.contributor.funderEuropean Commission
dc.contributor.funderEuropean Research Council
dc.contributor.funderFundación ProCNIC
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.description.peerreviewedes_ES
dc.identifier.e-issn1477-9129es_ES
dc.relation.publisherversionhttps://doi.org/10.1242/dev.174961es_ES
dc.identifier.journalDevelopment (Cambridge, England)es_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Desarrollo del Epicardio y su Papel en la Regeneraciónes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Señalización Intercelular durante el Desarrollo y la Enfermedad Cardiovasculares_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BFU2014–56970–Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/337703/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2016-78370-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CB16/11/00399es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD16/0011/0021es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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