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dc.contributor.authorGarcía-Romero, N
dc.contributor.authorMadurga, R
dc.contributor.authorRackov, G
dc.contributor.authorPalacín-Aliana, I
dc.contributor.authorNúñez-Torres, R
dc.contributor.authorAsensi-Puig, A
dc.contributor.authorCarrión-Navarro, J
dc.contributor.authorEsteban-Rubio, Susana
dc.contributor.authorPeinado Selgas, Hector 
dc.contributor.authorGonzález-Neira A, A 
dc.contributor.authorGonzález-Rumayor, V
dc.contributor.authorBelda-Iniesta, Cristobal 
dc.contributor.authorAyuso-Sacido, A
dc.date.accessioned2019-06-27T09:29:02Z
dc.date.available2019-06-27T09:29:02Z
dc.date.issued2019-03-11
dc.identifier.citationJ Transl Med. 201;17(1):75.es_ES
dc.identifier.issn1479-5876es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7808
dc.description.abstractBACKGROUND: Extracellular vesicles (EVs) are small membrane-bound vesicles which play an important role in cell-to-cell communication. Their molecular cargo analysis is presented as a new source for biomarker detection, and it might provide an alternative to traditional solid biopsies. However, the most effective approach for EV isolation is not yet well established. RESULTS: Here, we study the efficiency of the most common EV isolation methods-ultracentrifugation, Polyethlyene glycol and two commercial kits, Exoquick® and PureExo®. We isolated circulating EVs from the bloodstream of healthy donors, characterized the size and yield of EVs and analyzed their protein profiles and concentration. Moreover, we have used for the first time Digital-PCR to identify and detect specific gDNA sequences, which has several implications for diagnostic and monitoring many types of diseases. CONCLUSIONS: Our findings present Polyethylene glycol precipitation as the most feasible and less cost-consuming EV isolation technique.es_ES
dc.description.sponsorshipWe are grateful for the financial support from the ‘Fondo de Investigaciones Sanitarias’ (FIS) (PI14_00077), the Miguel Servet Program (CP11/00147) del Instituto de Salud Carlos III (AAS), and the Ministerio de Economía y Competi‑tividad–FEDERER (RTC‑2016‑4990‑1, RTC‑2015‑3846‑1). SER was supported by FPI‑CEU predoctoral fellowship.es_ES
dc.language.isoenges_ES
dc.publisherBMCes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectExoQuick®es_ES
dc.subjectExtracellular vesicleses_ES
dc.subjectLiquid biopsyes_ES
dc.subjectPolyethylene glycoles_ES
dc.subjectPureExo®es_ES
dc.subjectUltracentrifugationes_ES
dc.titlePolyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolationes_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID30871557es_ES
dc.format.volume17es_ES
dc.format.number1es_ES
dc.format.page75es_ES
dc.identifier.doi10.1186/s12967-019-1825-3es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.description.peerreviewedes_ES
dc.identifier.e-issn1479-5876es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/10.1186/s12967-019-1825-3.es_ES
dc.identifier.journalJournal of translational medicinees_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Microambiente y Metástasises_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI14/00077es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CP11/00147es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RTC-2016-4990-1es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RTC-2015-3846-1es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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