Mostrar el registro sencillo del ítem

dc.contributor.authorHurtado, Carolina 
dc.contributor.authorBustos, Maria Jose
dc.contributor.authorSabina, Prado
dc.contributor.authorNogal, Maria Luisa
dc.contributor.authorGranja, Aitor G
dc.contributor.authorGonzalez Portal, Maria Eugenia 
dc.contributor.authorGónzalez-Porqué, Pedro
dc.contributor.authorRevilla, Yolanda
dc.contributor.authorCarrascosa, Angel L
dc.date.accessioned2019-06-21T08:42:18Z
dc.date.available2019-06-21T08:42:18Z
dc.date.issued2008
dc.identifier.citationAntivir Ther. 2008;13(7):909-17es_ES
dc.identifier.issn1359-6535es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7797
dc.description.abstractBACKGROUND: Antiviral compounds are needed in the control of many animal and human diseases. METHODS: We analysed the effect of the antitumoural drug lauryl gallate on the infectivity of the African swine fever virus among other DNA (herpes simplex and vaccinia) and RNA (influenza, porcine transmissible gastroenteritis and Sindbis) viruses, paying attention to its effect on the viability of the corresponding host cells. RESULTS: Viral production was strongly inhibited in different cell lines at non-toxic concentrations of the drug (1-10 microM), reducing the titres 3->5 log units depending on the multiplicity of infection. In our model system (African swine fever virus in Vero cells), the addition of the drug 1 h before virus adsorption completely abolished virus productivity in a one-step growth virus cycle. Interestingly, no inhibitory effect was observed when lauryl gallate was added after 5-8 h post-infection. Both cellular and viral DNA synthesis and late viral transcription were inhibited by the drug; however, the early viral protein synthesis and the virus-mediated increase of p53 remained unaffected. Activation of the apoptotic effector caspase-3 was not detected after lauryl gallate treatment of Vero cells. Furthermore, the presence of the drug abrogated the activation of this protease induced by the virus infection. CONCLUSIONS: Lauryl gallate is a powerful antiviral agent against several pathogens of clinical and veterinary importance. The overall results indicate that a cellular factor or function might be the target of the antiviral action of alkyl gallates.es_ES
dc.description.sponsorshipThis work was supported by grants from Ministerio de Educación y Ciencia, Spain (BFU2004-00298/BMC), Laboratorios del Dr Esteve, Barcelona, Spain and by institutional grants from Fundación Ramón Areces, Madrid, Spain and Banco Central Hispano, Madrid, Spain. CH was a fellow from Fundación Ramón Areces. AGG was funded by Centro de Investigación en Sanidad Animal (CISA), Valdeolmos, Spain.es_ES
dc.language.isoenges_ES
dc.type.hasVersionAMes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectAntiviral activityes_ES
dc.subjectprotein kinaseses_ES
dc.subjectapoptosises_ES
dc.subject.meshAfrican Swine Fever Virus es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAntiviral Agents es_ES
dc.subject.meshCell Line es_ES
dc.subject.meshCercopithecus aethiopses_ES
dc.subject.meshCricetinae es_ES
dc.subject.meshDNA Viruses es_ES
dc.subject.meshDNA, Viral es_ES
dc.subject.meshGallic Acid es_ES
dc.subject.meshProteins es_ES
dc.subject.meshRNA Viruses es_ES
dc.subject.meshVero Cells es_ES
dc.subject.meshViral Proteins es_ES
dc.subject.meshVirus Replication es_ES
dc.titleAntiviral activity of lauryl gallate against animal viruseses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID19043925es_ES
dc.format.volume13es_ES
dc.format.number7es_ES
dc.format.page909-17es_ES
dc.contributor.funderEsteve (España) 
dc.contributor.funderMinisterio de Educación y Ciencia (España) 
dc.contributor.funderFundación Ramón Areces 
dc.contributor.funderBanco Central Hispano 
dc.contributor.funderCentro de Investigación en Sanidad Animal 
dc.description.peerreviewedNoes_ES
dc.identifier.journalAntiviral therapyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BFU2004-00298/BMCes_ES
dc.rights.accessRightsopen accesses_ES


Ficheros en el ítem

Acceso Abierto
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución-NoComercial-CompartirIgual 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución-NoComercial-CompartirIgual 4.0 Internacional