dc.contributor.author | Seguí, Raimundo | |
dc.contributor.author | Muñoz-Antoli, Carla | |
dc.contributor.author | Klisiowicz, Debora R | |
dc.contributor.author | Oishi, Camila Y | |
dc.contributor.author | Köster, Pamela Carolina | |
dc.contributor.author | Lucio, Aida de | |
dc.contributor.author | Hernandez-De-Mingo, Marta | |
dc.contributor.author | Puente, Paula | |
dc.contributor.author | Toledo, Rafael | |
dc.contributor.author | Esteban, José G | |
dc.contributor.author | Carmena, David | |
dc.date.accessioned | 2019-05-20T09:21:06Z | |
dc.date.available | 2019-05-20T09:21:06Z | |
dc.date.issued | 2018-08-30 | |
dc.identifier.citation | Parasit Vectors. 2018 Aug 30;11(1):490 | es_ES |
dc.identifier.issn | 1756-3305 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7597 | |
dc.description.abstract | BACKGROUND: Intestinal protozoan parasites are major contributors to the global burden of gastrointestinal disease causing significant socioeconomic consequences. Children living in resource-poor settings with restricted access to water and sanitary services are particularly at risk of these infections. METHODS: A prospective, community-based, cross-sectional survey was conducted in Paraná (southern Brazil) between May 2015 and May 2016. A total of 766 stool samples were individually collected from volunteers (male/female ratio: 0.99; age range: 0-76 years) and used for investigating the presence of intestinal helminth and protozoan species by routine microscopic procedures including the Kato-Katz and modified Ritchie concentration methods and the Ziehl-Neelsen stain technique. Quantitative real-time PCR confirmed microscopy-positive samples for Giardia duodenalis and the assemblages and sub-assemblages determined by multilocus sequence-based genotyping of the glutamate dehydrogenase (gdh) and β-giardin (bg) genes of the parasite. Identification of Blastocystis subtypes was carried out by amplification and sequencing of a partial fragment of the small-subunit ribosomal RNA gene (SSU rDNA) of this heterokont microorganism. RESULTS: Overall, 46.1% (353/766) of the participants were infected/colonised by at least one intestinal parasite/commensal species. Protozoan and helminth species were detected in 42.7% and 10.1% of the surveyed population, respectively. Blastocystis sp. (28.2%), Endolimax nana (14.9%), and Giardia duodenalis (11.0%) were the most prevalent species found among protozoans and Ascaris lumbricoides (5.0%), Trichuris trichiura (4.6%) and hookworms (1.0%) among helminths. A total of 38 G. duodenalis-positive samples were genotyped at gdh and bg markers, revealing the presence of the sub-assemblages AII (47.4%), AII/AIII (2.6%), BIII (5.3%), BIV (26.3%) and BIII/BIV (13.1%). Two samples (5.3%) were only identified as assemblage B. AII was predominantly found in females aged 5-9 years and was associated with a higher likelihood of reporting gastrointestinal symptoms. A total of 102 Blastocystis-positive samples were successfully subtyped at the SSU rRNA gene revealing the presence of ST1 (36.3%), ST2 (15.7%), ST3 (41.2%), ST4 (2.9%), ST6 (1.0%) and ST8 (2.9%). CONCLUSIONS: Data presented here indicate that enteric parasites still represent a pressing health concern in Paraná, Brazil, probably due to sub-optimal water, sanitation and hygiene conditions. A mostly anthroponotic origin is suspected for G. duodenalis and Blastocystis sp. infections. | es_ES |
dc.description.sponsorship | This study was funded by the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain) - Collaborative Research Network on Tropical Diseases (RICET) and the European Regional Development Fund (ERDF) under project RD12/0018/0013. Additional funding was also provided by the ISCIII under project CP12/03081 and by the Secretária Municipal de Saúde de Paranaguá (Paraná), Brazil. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central (BMC) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Brazil | es_ES |
dc.subject | Community | es_ES |
dc.subject | Genotyping | es_ES |
dc.subject | Helminth | es_ES |
dc.subject | Human | es_ES |
dc.subject | Intestinal parasites | es_ES |
dc.subject | Microscopy | es_ES |
dc.subject | Nematode | es_ES |
dc.subject | Protozoa | es_ES |
dc.subject | Soil-transmitted helminths | es_ES |
dc.subject.mesh | Adolescent | es_ES |
dc.subject.mesh | Adult | es_ES |
dc.subject.mesh | Aged | es_ES |
dc.subject.mesh | Blastocystis | es_ES |
dc.subject.mesh | Blastocystis Infections | es_ES |
dc.subject.mesh | Brazil | es_ES |
dc.subject.mesh | Child | es_ES |
dc.subject.mesh | Child, Preschool | es_ES |
dc.subject.mesh | Cross-Sectional Studies | es_ES |
dc.subject.mesh | DNA, Ribosomal | es_ES |
dc.subject.mesh | Feces | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Genetic Variation | es_ES |
dc.subject.mesh | Giardia lamblia | es_ES |
dc.subject.mesh | Giardiasis | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Infant | es_ES |
dc.subject.mesh | Infant, Newborn | es_ES |
dc.subject.mesh | Intestinal Diseases, Parasitic | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Middle Aged | es_ES |
dc.subject.mesh | Prevalence | es_ES |
dc.subject.mesh | Prospective Studies | es_ES |
dc.subject.mesh | Residence Characteristics | es_ES |
dc.subject.mesh | Surveys and Questionnaires | es_ES |
dc.subject.mesh | Young Adult | es_ES |
dc.title | Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 30165880 | es_ES |
dc.format.volume | 11 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 490 | es_ES |
dc.identifier.doi | 10.1186/s13071-018-3054-7 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1756-3305 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1186/s13071-018-3054-7 | es_ES |
dc.identifier.journal | Parasites & vectors | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |