dc.contributor.author | Toran, Jose Luis | |
dc.contributor.author | Lopez, Juan Antonio | |
dc.contributor.author | Gomes-Alves, Patricia | |
dc.contributor.author | Aguilar, Susana | |
dc.contributor.author | Torroja, Carlos | |
dc.contributor.author | Trevisan-Herraz, Marco | |
dc.contributor.author | Moscoso, Isabel | |
dc.contributor.author | Sebastião, Maria João | |
dc.contributor.author | Serra, Margarida | |
dc.contributor.author | Brito, Catarina | |
dc.contributor.author | Cruz, Francisco Miguel | |
dc.contributor.author | Sepulveda, Juan Carlos | |
dc.contributor.author | Abad, José Luis | |
dc.contributor.author | Galan-Arriola, Carlos | |
dc.contributor.author | Ibáñez, Borja | |
dc.contributor.author | Martinez, Fernando | |
dc.contributor.author | Fernández, María Eugenia | |
dc.contributor.author | Fernández-Avilés, Francisco | |
dc.contributor.author | Palacios, Itziar | |
dc.contributor.author | R-Borlado, Luis | |
dc.contributor.author | Vazquez, Jesus | |
dc.contributor.author | Alves, Paula M | |
dc.contributor.author | Bernad, Antonio | |
dc.date.accessioned | 2019-03-18T13:23:41Z | |
dc.date.available | 2019-03-18T13:23:41Z | |
dc.date.issued | 2019-03-15 | |
dc.identifier.citation | Sci Rep. 2019; 9(1):4647 | es_ES |
dc.identifier.issn | 2045-2322 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7352 | |
dc.description.abstract | Adult cardiac progenitor/stem cells (CPC/CSC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Assisted by complementary RNAseq analysis, we defined the fraction of the CPC proteome associable with specific functions by comparison with human bone marrow mesenchymal stem cells (MSC), the reference population for cell therapy, and human dermal fibroblasts (HDF), as a distant reference. Label-free proteomic analysis identified 526 proteins expressed differentially in CPC. iTRAQ analysis confirmed differential expression of a substantial proportion of those proteins in CPC relative to MSC, and systems biology analysis defined a clear overrepresentation of several categories related to enhanced angiogenic potential. The CPC plasma membrane compartment comprised 1,595 proteins, including a minimal signature of 167 proteins preferentially or exclusively expressed by CPC. CDH5 (VE-cadherin), OX2G (OX-2 membrane glycoprotein; CD200), GPR4 (G protein-coupled receptor 4), CACNG7 (calcium voltage-gated channel auxiliary subunit gamma 7) and F11R (F11 receptor; junctional adhesion molecule A; JAM-A; CD321) were selected for validation. Their differential expression was confirmed both in expanded CPC batches and in early stages of isolation, particularly when compared against cardiac fibroblasts. Among them, GPR4 demonstrated the highest discrimination capacity between all cell lineages analyzed. | es_ES |
dc.description.sponsorship | This study was supported by funding from the European Commission (HEALTH-2009_242038), and by grants to AB from the Spanish Ministry of Science and Innovation (SAF2012-34327; SAF2015-70882-R), the Research Program of the Comunidad Autónoma de Madrid (S2011/BMD-2420), the Instituto de Salud Carlos III (RETICS-RD12/0019/0018), and grants from the Portuguese Foundation for Science and Technology (PTDC/ BBB-BIO/1414) to PMA. iNOVA4Health - UID/Multi/04462/2013, financially supported by FCT/Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is also acknowledged. JL Abad, I Palacios and LR Borlado were employees of Coretherapix; Coretherapix is part of Tigenix Group since July 2015. The other authors declare no conflict of interest. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 30874584 | es_ES |
dc.format.volume | 9 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 4647 | es_ES |
dc.identifier.doi | 10.1038/s41598-019-39571-x | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea | |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Fundação para a Ciência e Tecnologia (Portugal) | |
dc.contributor.funder | Ministério da Educação e Ciência (Portugal) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2045-2322 | es_ES |
dc.identifier.journal | Scientific reports | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Proteómica / Metabolómica | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Proteómica cardiovascular | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Antiguos CNIC | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Laboratorio Traslacional para la Imagen y Terapia Cardiovascular | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/242038/EU | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2012-34327 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2015-70882-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD12/0019/0018 | es_ES |
dc.rights.accessRights | open access | es_ES |