Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/7352
Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
Toran, Jose Luis CNIC | Lopez, Juan Antonio CNIC | Gomes-Alves, Patricia | Aguilar, Susana CNIC | Torroja, Carlos CNIC | Trevisan-Herraz, Marco CNIC | Moscoso, Isabel CNIC | Sebastião, Maria João | Serra, Margarida | Brito, Catarina | Cruz, Francisco Miguel CNIC | Sepulveda, Juan Carlos CNIC | Abad, José Luis | Galan-Arriola, Carlos CNIC | Ibanez, Borja CNIC | Martinez, Fernando CNIC | Fernández, María Eugenia | Fernández-Avilés, Francisco | Palacios, Itziar | R-Borlado, Luis | Vazquez, Jesus CNIC | Alves, Paula M | Bernad, Antonio CNIC
Sci Rep. 2019; 9(1):4647
Adult cardiac progenitor/stem cells (CPC/CSC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Assisted by complementary RNAseq analysis, we defined the fraction of the CPC proteome associable with specific functions by comparison with human bone marrow mesenchymal stem cells (MSC), the reference population for cell therapy, and human dermal fibroblasts (HDF), as a distant reference. Label-free proteomic analysis identified 526 proteins expressed differentially in CPC. iTRAQ analysis confirmed differential expression of a substantial proportion of those proteins in CPC relative to MSC, and systems biology analysis defined a clear overrepresentation of several categories related to enhanced angiogenic potential. The CPC plasma membrane compartment comprised 1,595 proteins, including a minimal signature of 167 proteins preferentially or exclusively expressed by CPC. CDH5 (VE-cadherin), OX2G (OX-2 membrane glycoprotein; CD200), GPR4 (G protein-coupled receptor 4), CACNG7 (calcium voltage-gated channel auxiliary subunit gamma 7) and F11R (F11 receptor; junctional adhesion molecule A; JAM-A; CD321) were selected for validation. Their differential expression was confirmed both in expanded CPC batches and in early stages of isolation, particularly when compared against cardiac fibroblasts. Among them, GPR4 demonstrated the highest discrimination capacity between all cell lineages analyzed.