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dc.contributor.authorMéndez, Jairo A
dc.contributor.authorUsme-Ciro, José A
dc.contributor.authorDomingo Carrasco, Cristina 
dc.contributor.authorRey, Gloria J
dc.contributor.authorSánchez, Juan A
dc.contributor.authorTenorio, Antonio 
dc.contributor.authorGallego-Gomez, Juan C
dc.identifier.citationVirol J. 2012 Mar 9;9:64.es_ES
dc.description.abstractBACKGROUND: Dengue fever is perhaps the most important viral re-emergent disease especially in tropical and sub-tropical countries, affecting about 50 million people around the world yearly. In Colombia, dengue virus was first detected in 1971 and still remains as a major public health issue. Although four viral serotypes have been recurrently identified, dengue virus type 2 (DENV-2) has been involved in the most important outbreaks during the last 20 years, including 2010 when the fatality rate highly increased. As there are no major studies reviewing virus origin and genotype distribution in this country, the present study attempts to reconstruct the phylogenetic history of DENV-2 using a sequence analysis from a 224 bp PCR-amplified product corresponding to the carboxyl terminus of the envelope (E) gene from 48 Colombian isolates. RESULTS: As expected, the oldest isolates belonged to the American genotype (subtype V), but the strains collected since 1990 represent the American/Asian genotype (subtype IIIb) as previously reported in different American countries. Interestingly, the introduction of this genotype coincides with the first report of dengue hemorrhagic fever in Colombia at the end of 1989 and the increase of cases during the next years. CONCLUSION: After replacement of the American genotype, several lineages of American/Asian subtype have rapidly spread all over the country evolving in new clades. Nevertheless, the direct association of these new variants in the raise of lethality rate observed during the last outbreak has to be demonstrated.es_ES
dc.description.sponsorshipThis research was supported by Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología Francisco José de Caldas-COLCIENCIAS grants 11150416336 CT 234-2004, 11150418079 and 111540820511 from the Colombian government and the Instituto Nacional de Salud resources.es_ES
dc.publisherBioMed Central (BMC) es_ES
dc.subject.meshColombia es_ES
dc.subject.meshDengue es_ES
dc.subject.meshDengue Virus es_ES
dc.subject.meshGenotype es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMolecular Epidemiology es_ES
dc.subject.meshRNA, Viral es_ES
dc.subject.meshViral Envelope Proteins es_ES
dc.subject.meshPhylogeny es_ES
dc.titlePhylogenetic reconstruction of dengue virus type 2 in Colombiaes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderGobierno de Colombia 
dc.contributor.funderInstituto Colombiano para el Desarrollo de la Ciencia y la Tecnología Francisco José de Caldas (Colombia) 
dc.contributor.funderInstituto Nacional de Salud (Colombia) 
dc.identifier.journalVirology journales_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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