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dc.contributor.authorGonçalves, Samuel M
dc.contributor.authorLagrou, Katrien
dc.contributor.authorRodrigues, Cláudia S
dc.contributor.authorCampos, Cláudia F
dc.contributor.authorBernal-Martinez, Leticia 
dc.contributor.authorRodrigues, Fernando
dc.contributor.authorSilvestre, Ricardo
dc.contributor.authorAlcazar-Fuoli, Laura 
dc.contributor.authorMaertens, Johan A
dc.contributor.authorCunha, Cristina
dc.contributor.authorCarvalho, Agostinho
dc.identifier.citationFront Microbiol. 2017 Nov 29;8:2362.es_ES
dc.description.abstractBackground: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced resolution. Methods: To determine whether a signature of alveolar cytokines could be associated with the development of IPA and used as a diagnostic biomarker, we performed a nested case-control study involving 113 patients at-risk. Results: Among the 32 analytes tested, IL-1β, IL-6, IL-8, IL-17A, IL-23, and TNFα were significantly increased among patients with IPA, defining two clusters able to accurately differentiate cases of infection from controls. Genetic variants previously reported to confer increased risk of IPA compromised the production of specific cytokines and impaired their discriminatory potential toward infection. Collectively, our data indicated that IL-8 was the best performing cytokine, with alveolar levels ≥904 pg/mL predicting IPA with elevated sensitivity (90%), specificity (73%), and negative predictive value (88%). Conclusions: These findings highlight the existence of a specific profile of alveolar cytokines, with IL-8 being the dominant discriminator, which might be useful in supporting current diagnostic approaches for IPA.es_ES
dc.description.sponsorshipFunding. This work was supported by a Mérieux Research Grant 2016 from Institut Mérieux; the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) (NORTE-01-0145-FEDER-000013); the Fundação para a Ciência e Tecnologia (FCT) (IF/00735/2014 and SFRH/BPD/96176/2013); the Spanish Ministry of Economy and Competitiveness, Instituto de Salud Carlos III (MPY 1277/15) (co-financed by the European Development Regional Fund “A Way to Achieve Europe”); and the Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015).es_ES
dc.publisherFrontiers Media es_ES
dc.subjectBronchoalveolar lavagees_ES
dc.subjectFungal diagnosticses_ES
dc.subjectInvasive pulmonary aspergillosises_ES
dc.titleEvaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patientses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstitut Mérieux 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderFundação para a Ciência e Tecnologia (Portugal) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.identifier.journalFrontiers in microbiologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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