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dc.contributor.authorRoiz, David
dc.contributor.authorVazquez, Ana 
dc.contributor.authorSanchez-Seco, Paz 
dc.contributor.authorTenorio, Antonio 
dc.contributor.authorRizzoli, Annapaola
dc.identifier.citationVirol J. 2009 Jul 5;6:93.es_ES
dc.description.abstractThe presence of DNA sequences integrated from a new flavivirus related to Cell Fusing Agent and Kamiti River Virus was identified in wild Aedes albopictus mosquito populations from the provinces of Trentino and Padova, Northern Italy. Field work was developed during August-October 2007 with BG-traps, and mosquitoes were screened for flavivirus and alphavirus. No alphavirus was detected, indicating that Chikungunya virus is not present in these mosquitoes in Trentino and Padova area. However, 21% of the pools were positive for flavivirus, further recognised with BLAST as similar to Kamiti River Virus. Phylogenetical analysis with 708 nucleotides from the NS5 gene identified this virus as a new member of the insect flavivirus clade, together with others like Kamiti River Virus, Cell Fusing Agent or Culex flavivirus, and in the group of those transmitted by Aedes. Furthermore, the treatment with RNAse, indicated that this flavivirus should be integrated in the genome of Ae. albopictus. These results propose that these sequences are transmitted by both sexes, and with different prevalence in the studied populations, and support the idea of a widespread distribution of integrated genomes in several mosquitoes from different areas, as first demonstrated with Cell Silent Agent. Evolutionary implications of this discovery and application in flavivirus phylogeny are discussed.es_ES
dc.description.sponsorshipThis work was supported by funding from the Autonomous Province of Trento for the postdoctoral project RISKTIGER: Risk assessment of new arbovirus diseases transmitted by Aedes albopictus (Diptera: Culicidae) in the Autonomous Province of Trento. Principal Investigator: David Roiz. This study was also supported in part by the Spanish Ministry of Science and Innovation and the Instituto de Salud Carlos III within the network of Tropical diseases Research (RICET RD06/0021).es_ES
dc.publisherBiomed Centrales_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshAedes es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCluster Analysis es_ES
dc.subject.meshDNA, Viral es_ES
dc.subject.meshFlavivirus es_ES
dc.subject.meshItaly es_ES
dc.subject.meshPhylogeny es_ES
dc.subject.meshProviruses es_ES
dc.subject.meshSequence Analysis, DNA es_ES
dc.subject.meshSequence Homology es_ES
dc.subject.meshViral Nonstructural Proteins es_ES
dc.titleDetection of novel insect flavivirus sequences integrated in Aedes albopictus (Diptera: Culicidae) in Northern Italyes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.identifier.journalVirology journales_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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Atribución 4.0 Internacional
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