Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/7104
Genetic diversity of HA1 domain of heammaglutinin gene of influenza A(H1N1)pdm09 in Tunisia
El Moussi, Awatef | Ben Hadj Kacem, Mohamed Ali | Pozo Sanchez, Francisco ISCIII | Ledesma, Juan ISCIII | Cuevas, Maria Teresa ISCIII | Casas Flecha, Inmaculada ISCIII | Slim, Amine
Virol J. 2013 May 16;10:150.
We present major results concerning isolation and determination of the nucleotide sequence of hemagglutinin (HA1) of the pandemic (H1N1)pdm09 influenza viruses found in Tunisia. Amino acid analysis revealed minor amino acid changes in the antigenic or receptor-binding domains. We found mutations that were also present in 1918 pandemic virus, which includes S183P in 4 and S185T mutation in 19 of 27 viruses analyzed from 2011, while none of the 2009 viruses carried these mutations. Also two specific amino acid differences into N-glycosylation sites (N288T and N276H) were detected. The phylogenetic analysis revealed that the majority of the Tunisian isolates clustered with clade A/St. Petersburg/27/2011 viruses characterized by D97N and S185T mutations. However it also reveals a trend of 2010 strains to accumulate amino acid variation and form new phylogenetic clade with three specific amino acid substitutions: V47I, E172K and K308E.
Amino Acid Substitution | Cluster Analysis | Hemagglutinin Glycoproteins, Influenza Virus | Humans | Influenza A Virus, H1N1 Subtype | Influenza, Human | Molecular Sequence Data | Phylogeny | RNA, Viral | Sequence Analysis, DNA | Sequence Homology, Amino Acid | Tunisia | Genetic Variation
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