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dc.contributor.authorAdell-Aledón, Manuel
dc.contributor.authorKöster, Pamela Carolina 
dc.contributor.authorLucio, Aida de 
dc.contributor.authorPuente, Paula 
dc.contributor.authorHernandez-De-Mingo, Marta 
dc.contributor.authorSánchez-Thevenet, Paula
dc.contributor.authorDea-Ayuela, María Auxiliadora
dc.contributor.authorCarmena, David
dc.identifier.citationBMC Vet Res. 2018 Jan 22;14(1):26es_ES
dc.description.abstractBACKGROUND: Giardia duodenalis is one of the most common enteric parasites in domestic animals including dogs. Young animals are more prone to the infection, with clinical manifestations ranging from asymptomatic to acute or chronic diarrhoea. Dogs are primarily infected by canine-specific (C-D) assemblages of G. duodenalis. However, zoonotic assemblages A and B have been increasingly documented in canine isolates, raising the question of whether and to which extent dogs can act as natural reservoirs of human giardiosis. METHODS: In this cross-sectional epidemiological survey we assessed the molecular diversity of G. duodenalis in dogs in the province of Castellón, Eastern Spain. A total of 348 individual faecal samples from sheltered (n = 218), breeding (n = 24), hunting (n = 68), shepherd (n = 24), and pet (n = 14) dogs were collected between 2014 and 2016. Detection of G. duodenalis cysts in faecal material was carried out by direct fluorescence microscopy as a screening test, whereas a qPCR targeting the small subunit ribosomal RNA gene of the parasite was subsequently used as a confirmatory method. RESULTS: Giardia duodenalis was detected in 36.5% (95% CI: 31.6-41.7%) of dogs. No significant differences in prevalence rates could be demonstrated among dogs according to their sex and geographical origin, but breeding (45.8%; 95% CI: 27.9-64.9%) and sheltered (40.4%; 95% CI: 34.1-47.0%) dogs harboured significantly higher proportions of G. duodenalis. Multi-locus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of G. duodenalis allowed the characterization of 35 canine isolates that were unambiguously assigned to assemblages A (14.3%), B (22.9%), C (5.7%), and D (37.1%). A number of inter-assemblage mixed infections including A + B (11.4%), A + D (2.9%), and A + B + D (5.7%) were also identified. CONCLUSIONS: Data presented here are strongly indicative of high infection pressures in kennelled animals. Zoonotic sub-assemblages AII, BIII, and BIV were responsible for a considerable proportion of the G. duodenalis infections detected, but very few of the genotypes identified have been previously documented in Spanish human populations. Although possible, zoonotic transmission between dogs and humans seems an infrequent event in this Spanish region.es_ES
dc.description.sponsorshipThis study was funded by the Health Institute Carlos III, Ministry of Economy and Competitiveness under project CP12/03081. Additional funding was obtained from the CEU-Santander/Zoocan Study.es_ES
dc.publisherBioMed Central (BMC) es_ES
dc.subjectGiardia duodenalises_ES
dc.subjectMolecular epidemiologyes_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCross-Sectional Studies es_ES
dc.subject.meshDog Diseases es_ES
dc.subject.meshDogs es_ES
dc.subject.meshFeces es_ES
dc.subject.meshFemale es_ES
dc.subject.meshGenes, rRNA es_ES
dc.subject.meshGiardia lamblia es_ES
dc.subject.meshGiardiasis es_ES
dc.subject.meshMale es_ES
dc.subject.meshMicroscopy, Fluorescence es_ES
dc.subject.meshMolecular Epidemiology es_ES
dc.subject.meshSpain es_ES
dc.titleOccurrence and molecular epidemiology of Giardia duodenalis infection in dog populations in eastern Spaines_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.identifier.journalBMC veterinary researches_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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