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dc.contributor.authorFernández-Soto, Pedro
dc.contributor.authorSánchez-Hernández, Alicia
dc.contributor.authorGandasegui, Javier
dc.contributor.authorBajo Santos, Cristina
dc.contributor.authorLópez-Abán, Julio
dc.contributor.authorSaugar, Jose Maria 
dc.contributor.authorRodriguez, Esperanza 
dc.contributor.authorVicente, Belén
dc.contributor.authorMuro, Antonio
dc.date.accessioned2019-01-10T11:19:15Z
dc.date.available2019-01-10T11:19:15Z
dc.date.issued2016-07-14
dc.identifier.citationPLoS Negl Trop Dis. 2016 Jul 14;10(7):e0004836.es_ES
dc.identifier.issn1935-2735es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6985
dc.description.abstractBACKGROUND: Strongyloides stercoralis, the chief causative agent of human strongyloidiasis, is a nematode globally distributed but mainly endemic in tropical and subtropical regions. Chronic infection is often clinically asymptomatic but it can result in severe hyperinfection syndrome or disseminated strongyloidiasis in immunocompromised patients. There is a great diversity of techniques used in diagnosing the disease, but definitive diagnosis is accomplished by parasitological examination of stool samples for morphological identification of parasite. Until now, no molecular method has been tested in urine samples as an alternative to stool samples for diagnosing strongyloidiasis. This study aimed to evaluate the use of a new molecular LAMP assay in a well-established Wistar rat experimental infection model using both stool and, for the first time, urine samples. The LAMP assay was also clinically evaluated in patients´ stool samples. METHODOLOGY/PRINCIPAL FINDINGS: Stool and urine samples were obtained daily during a 28-day period from rats infected subcutaneously with different infective third-stage larvae doses of S. venezuelensis. The dynamics of parasite infection was determined by daily counting the number of eggs per gram of feces from day 1 to 28 post-infection. A set of primers for LAMP assay based on a DNA partial sequence in the 18S rRNA gene from S. venezuelensis was designed. The set up LAMP assay (namely, Strong-LAMP) allowed the sensitive detection of S. venezuelensis DNA in both stool and urine samples obtained from each infection group of rats and was also effective in S. stercoralis DNA amplification in patients´ stool samples with previously confirmed strongyloidiasis by parasitological and real-time PCR tests. CONCLUSIONS/SIGNIFICANCE: Our Strong-LAMP assay is an useful molecular tool in research of a strongyloidiasis experimental infection model in both stool and urine samples. After further validation, the Strong-LAMP could also be potentially applied for effective diagnosis of strongyloidiasis in a clinical setting.es_ES
dc.description.sponsorshipThis work was supported by Real Federación Española de Fútbol-Sociedad Española de Medicina Tropical y Salud Internacional, 2013 (RFEF-SEMTSI, 2013) and by Junta de Castilla y León (Ref. no. SA342U13). This study was also partially supported by the 6th National Plan (PN) of Research + Development + Innovation (I+D+I) 2008–2011, Carlos III Health Institute (ISCIII) General Division Networks and Cooperative Research Centres + Collaborative Research Network on Tropical Diseases (RICET) (RD12/0018/0011). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnimals es_ES
dc.subject.meshDNA Primers es_ES
dc.subject.meshDNA, Helminth es_ES
dc.subject.meshFeces es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMale es_ES
dc.subject.meshNucleic Acid Amplification Techniques es_ES
dc.subject.meshRats es_ES
dc.subject.meshRats, Wistar es_ES
dc.subject.meshStrongyloides stercoralis es_ES
dc.subject.meshStrongyloidiasis es_ES
dc.subject.meshUrine es_ES
dc.titleStrong-LAMP: A LAMP Assay for Strongyloides spp. Detection in Stool and Urine Samples. Towards the Diagnosis of Human Strongyloidiasis Starting from a Rodent Modeles_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID27415764es_ES
dc.format.volume10es_ES
dc.format.number7es_ES
dc.format.pagee0004836es_ES
dc.identifier.doi10.1371/journal.pntd.0004836es_ES
dc.contributor.funderReal Federación Española de Fútbol 
dc.contributor.funderJunta de Castilla y León (España) 
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderRETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España) 
dc.contributor.funderSociedad Española de Medicina Tropical y Salud Internacional 
dc.contributor.funderPlan Nacional de I+D+i (España) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1935-2735es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pntd.0004836es_ES
dc.identifier.journalPLoS neglected tropical diseaseses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0018/0011es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional