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dc.contributor.author | Sanchez-Del Cojo, Maria | |
dc.contributor.author | Díez-Fuertes, Francisco | |
dc.contributor.author | Rodriguez‑Mora, Sara | |
dc.contributor.author | Bermejo, Mercedes | |
dc.contributor.author | López-Campos, Guillermo | |
dc.contributor.author | Mateos, Elena | |
dc.contributor.author | Jiménez-Tormo, Laura | |
dc.contributor.author | Gómez-Esquer, Francisco | |
dc.contributor.author | Díaz-Gil, Gema | |
dc.contributor.author | Alcamí, José | |
dc.contributor.author | Coiras, Mayte | |
dc.contributor.author | Lopez-Huertas, Maria Rosa | |
dc.date.accessioned | 2018-12-19T12:15:35Z | |
dc.date.available | 2018-12-19T12:15:35Z | |
dc.date.issued | 2017-10-02 | |
dc.identifier.citation | PLoS One. 2017 Oct 2;12(10):e0185677. | es_ES |
dc.identifier.issn | 1932-6203 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6908 | |
dc.description.abstract | HIV-1 induces changes in the miRNA expression profile of infected CD4+ T cells that could improve viral replication. HIV-1 regulator Tat modifies the cellular gene expression and has been appointed as an RNA silencing suppressor. Tat is a 101-residue protein codified by two exons that regulates the elongation of viral transcripts. The first exon of Tat (amino acids 1-72) forms the transcriptionally active protein Tat72, but the presence of the second exon (amino acids 73-101) results in a more competent regulatory protein (Tat101) with additional functions. Intracellular, full-length Tat101 induces functional and morphological changes in CD4+ T cells that contribute to HIV-1 pathogenesis such as delay in T-cell proliferation and protection against FasL-mediated apoptosis. But the precise mechanism by which Tat produces these changes remains unknown. We analyzed how the stable expression of intracellular Tat101 and Tat72 modified the miRNA expression profile in Jurkat cells and if this correlated with changes in apoptotic pathways and cell cycle observed in Tat-expressing cells. Specifically, the enhanced expression of hsa-miR-21 and hsa-miR-222 in Jurkat-Tat101 cells was associated with the reduced expression of target mRNAs encoding proteins related to apoptosis and cell cycle such as PTEN, PDCD4 and CDKN1B. We developed Jurkat cells with stable expression of hsa-miR-21 or hsa-miR-222 and observed a similar pattern to Jurkat-Tat101 in resistance to FasL-mediated apoptosis, cell cycle arrest in G2/M and altered cell morphology. Consequently, upregulation of hsa-miR-21 and hsa-miR-222 by Tat may contribute to protect against apoptosis and to anergy observed in HIV-infected CD4+ T cells. | es_ES |
dc.description.sponsorship | This work was supported by the Spanish Ministry of Economy and Competitiveness (SAF2010-18388, SAF2013-44677-R, PI12/00506, PIE 13/00040); the Spanish AIDS Research Network RD12/0017/0015 that is included in the Spanish I+D+I Plan and is co-financed by ISCIII-Subdirección General de Evaluacion and European Funding for Regional Development (FEDER). The work of María Sánchez del Cojo is supported by a fellowship of Fondo de Investigaciones Sanitarias (FI09/00347). The Spanish Ministry of Economy and Competitiveness supported the work of María Rosa López-Huertas (PIE 13/00040), Elena Mateos (SAF2010-18388), Sara Rodríguez-Mora (Sara Borrell fellowship) and Francisco Díez-Fuertes (Sara Borrell fellowship). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (PLOS) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | CD4-Positive T-Lymphocytes | es_ES |
dc.subject.mesh | Gene Expression Profiling | es_ES |
dc.subject.mesh | Gene Products, tat | es_ES |
dc.subject.mesh | Genetic Vectors | es_ES |
dc.subject.mesh | HIV-1 | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Jurkat Cells | es_ES |
dc.subject.mesh | MicroRNAs | es_ES |
dc.subject.mesh | Oligonucleotide Array Sequence Analysis | es_ES |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | es_ES |
dc.subject.mesh | Up-Regulation | es_ES |
dc.subject.mesh | Apoptosis | es_ES |
dc.subject.mesh | Cell Proliferation | es_ES |
dc.title | Changes in the cellular microRNA profile by the intracellular expression of HIV-1 Tat regulator: A potential mechanism for resistance to apoptosis and impaired proliferation in HIV-1 infected CD4+ T cells | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 28968466 | es_ES |
dc.format.volume | 12 | es_ES |
dc.format.number | 10 | es_ES |
dc.format.page | e0185677 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0185677 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Red de Investigación Cooperativa en Investigación en Sida (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1932-6203 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0185677 | es_ES |
dc.identifier.journal | PloS one | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2010-18388 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2013-44677-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PI12/00506 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PIE 13/00040 | es_ES |
dc.rights.accessRights | open access | es_ES |