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dc.contributor.authorDelgado, Elena 
dc.contributor.authorCuevas, Maria Teresa 
dc.contributor.authorDominguez, Francisco 
dc.contributor.authorVega Rocha, Yolanda 
dc.contributor.authorCabello, Marina
dc.contributor.authorFernandez-Garcia, Aurora 
dc.contributor.authorPérez-Losada, Marcos
dc.contributor.authorCastro, María Ángeles
dc.contributor.authorMontero, Vanessa 
dc.contributor.authorSánchez, Mónica
dc.contributor.authorMariño, Ana
dc.contributor.authorÁlvarez, Hortensia
dc.contributor.authorOrdóñez, Patricia
dc.contributor.authorOcampo, Antonio
dc.contributor.authorMiralles, Celia
dc.contributor.authorPérez-Castro, Sonia
dc.contributor.authorLópez-Álvarez, María José
dc.contributor.authorRodríguez, Raúl
dc.contributor.authorTrigo, Matilde
dc.contributor.authorDiz-Arén, Julio
dc.contributor.authorHinojosa, Carmen
dc.contributor.authorBachiller, Pablo
dc.contributor.authorHernáez-Crespo, Silvia
dc.contributor.authorCisterna, Ramón
dc.contributor.authorGarduño, Eugenio
dc.contributor.authorPerez-Alvarez, Lucia 
dc.contributor.authorThomson, Michael M
dc.identifier.citationPLoS One. 2015 Nov 24;10(11):e0143325es_ES
dc.description.abstractWe recently reported the rapid expansion of an HIV-1 subtype F cluster among men who have sex with men (MSM) in the region of Galicia, Northwest Spain. Here we update this outbreak, analyze near full-length genomes, determine phylogenetic relationships, and estimate its origin. For this study, we used sequences of HIV-1 protease-reverse transcriptase and env V3 region, and for 17 samples, near full-length genome sequences were obtained. Phylogenetic analyses were performed via maximum likelihood. Locations and times of most recent common ancestors were estimated using Bayesian inference. Among samples analyzed by us, 100 HIV-1 F1 subsubtype infections of monophyletic origin were diagnosed in Spain, including 88 in Galicia and 12 in four other regions. Most viruses (n = 90) grouped in a subcluster (Galician subcluster), while 7 from Valladolid (Central Spain) grouped in another subcluster. At least 94 individuals were sexually-infected males and at least 71 were MSM. Seventeen near full-length genomes were uniformly of F1 subsubtype. Through similarity searches and phylogenetic analyses, we identified 18 viruses from four other Western European countries [Switzerland (n = 8), Belgium (n = 5), France (n = 3), and United Kingdom (n = 2)] and one from Brazil, from samples collected in 2005-2011, which branched within the subtype F cluster, outside of both Spanish subclusters, most of them corresponding to recently infected individuals. The most probable geographic origin and age of the Galician subcluster was Ferrol, Northwest Galicia, around 2007, while the Western European cluster probably emerged in Switzerland around 2002. In conclusion, a recently expanded HIV-1 subtype F cluster, the largest non-subtype B cluster reported in Western Europe, continues to spread among MSM in Spain; this cluster is part of a larger cluster with a wide geographic circulation in diverse Western European countries.es_ES
dc.description.sponsorshipThis work received support from the Dirección General de Farmacia, Ministerio de Sanidad, Servicios Sociales e Igualdad, Government of Spain, grant EC11-272; European Network of Excellence EUROPRISE (Rational Design of HIV Vaccines and Microbicides), grant LSHP-CT-2006-037611; European Research Infrastructures for Poverty Related Diseases (EURIPRED). Seventh Framework Programme: FP7-Capacities-infrastructures-2012-1, grant agreement 312661; Instituto de Salud Carlos III, Subdirección General de Evaluación, and Fondo Europeo de Desarrollo Regional (FEDER), Plan Nacional I + D + I, through project RD12/0017/0026; Consellería de Sanidade, Government of Galicia, Spain (MVI 1291/08); and the Osakidetza-Servicio Vasco de Salud, Basque Country, Spain (MVI-1255-08). Marcos Pérez-Losada was supported by a DC D-CFAR Research Award from the District of Columbia Developmental Center for AIDS Research (P30AI087714) and by an University Facilitating Fund award from George Washington University. Aurora Fernández-García is supported by CIBER in Epidemiology and Public Health, Instituto de Salud Carlos III, Madrid, Spain.es_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshAntiretroviral Therapy, Highly Activees_ES
dc.subject.meshBayes Theorem es_ES
dc.subject.meshDisease Outbreaks es_ES
dc.subject.meshDrug Resistance, Viral es_ES
dc.subject.meshEurope es_ES
dc.subject.meshGenotype es_ES
dc.subject.meshHIV Infections es_ES
dc.subject.meshHIV-1 es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMale es_ES
dc.subject.meshMolecular Sequence Data es_ES
dc.subject.meshPrevalence es_ES
dc.subject.meshSpain es_ES
dc.subject.meshHomosexuality, Male es_ES
dc.subject.meshPhylogeny es_ES
dc.subject.meshPhylogeography es_ES
dc.titlePhylogeny and Phylogeography of a Recent HIV-1 Subtype F Outbreak among Men Who Have Sex with Men in Spain Deriving from a Cluster with a Wide Geographic Circulation in Western Europees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Sanidad, Servicios Sociales e Igualdad (España)
dc.contributor.funderUnión Europea. Comisión Europea. 7 Programa Marco
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderXunta de Galicia
dc.contributor.funderGobierno Vasco
dc.contributor.funderEuropean Network of Excellence (EUROPRISE)
dc.contributor.funderEuropean Research Infrastructures for Poverty Related Diseases (EURIPRED)
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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