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dc.contributor.author | Gottstein, Bruno | |
dc.contributor.author | Schneeberger, Marianne | |
dc.contributor.author | Boubaker, Ghalia | |
dc.contributor.author | Merkle, Bernadette | |
dc.contributor.author | Huber, Cristina | |
dc.contributor.author | Spiliotis, Markus | |
dc.contributor.author | Müller, Norbert | |
dc.contributor.author | Garate, Teresa | |
dc.contributor.author | Doherr, Marcus G | |
dc.date.accessioned | 2018-12-19T10:44:33Z | |
dc.date.available | 2018-12-19T10:44:33Z | |
dc.date.issued | 2014-06-12 | |
dc.identifier.citation | PLoS Negl Trop Dis. 2014 Jun 12;8(6):e2860 | es_ES |
dc.identifier.issn | 1935-2735 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6899 | |
dc.description.abstract | Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2) and cathepsin L-1 (recCL1), were assessed individually and in combination in enzyme-linked immunosorbent assays (ELISA) for the specific serodiagnosis of human fasciolosis in areas of low endemicity as encountered in Central Europe. Antibody detection was conducted using ProteinA/ProteinG (PAG) conjugated to alkaline phosphatase. Test characteristics as well as agreement with results from an ELISA using excretory-secretory products (FhES) from adult stage liver flukes was assessed by receiver operator characteristic (ROC) analysis, specificity, sensitivity, Youdens J and overall accuracy. Cross-reactivity was assessed using three different groups of serum samples from healthy individuals (n=20), patients with other parasitic infections (n=87) and patients with malignancies (n=121). The best combined diagnostic results for recombinant antigens were obtained using the recSAP2-ELISA (87% sensitivity, 99% specificity and 97% overall accuracy) employing the threshold (cut-off) to discriminate between positive and negative reactions that maximized Youdens J. The findings showed that recSAP2-ELISA can be used for the routine serodiagnosis of chronic fasciolosis in clinical laboratories; the use of the PAG-conjugate offers the opportunity to employ, for example, rabbit hyperimmune serum for the standardization of positive controls. | es_ES |
dc.description.sponsorship | The authors received no specific funding for this study. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (PLOS) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Adolescent | es_ES |
dc.subject.mesh | Adult | es_ES |
dc.subject.mesh | Aged | es_ES |
dc.subject.mesh | Aged, 80 and over | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Antibodies, Helminth | es_ES |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | es_ES |
dc.subject.mesh | Europe | es_ES |
dc.subject.mesh | Fasciola hepatica | es_ES |
dc.subject.mesh | Fascioliasis | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Middle Aged | es_ES |
dc.subject.mesh | Recombinant Proteins | es_ES |
dc.subject.mesh | Sensitivity and Specificity | es_ES |
dc.subject.mesh | Serologic Tests | es_ES |
dc.subject.mesh | Young Adult | es_ES |
dc.subject.mesh | Antigens, Helminth | es_ES |
dc.title | Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 24922050 | es_ES |
dc.format.volume | 8 | es_ES |
dc.format.number | 6 | es_ES |
dc.format.page | e2860 | es_ES |
dc.identifier.doi | 10.1371/journal.pntd.0002860 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1935-2735 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pntd.0002860 | es_ES |
dc.identifier.journal | PLoS neglected tropical diseases | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |