Show simple item record

dc.contributor.authorFerrandiz-Avellano, Maria-Jose 
dc.contributor.authorArnanz, Cristina 
dc.contributor.authorMartin-Galiano, Antonio Javier 
dc.contributor.authorRodriguez-Martin, Carlos 
dc.contributor.authorde la Campa, Adela G
dc.identifier.citationPLoS One. 2014 Jul 14;9(7):e101574es_ES
dc.description.abstractThe most basic level of transcription regulation in Streptococcus pneumoniae is the organization of its chromosome in topological domains. In response to drugs that caused DNA-relaxation, a global transcriptional response was observed. Several chromosomal domains were identified based on the transcriptional response of their genes: up-regulated (U), down-regulated (D), non-regulated (N), and flanking (F). We show that these distinct domains have different expression and conservation characteristics. Microarray fluorescence units under non-relaxation conditions were used as a measure of gene transcriptional level. Fluorescence units were significantly lower in F genes than in the other domains with a similar AT content. The transcriptional level of the domains categorized them was D>U>F. In addition, a comparison of 12 S. pneumoniae genome sequences showed a conservation of gene composition within U and D domains, and an extensive gene interchange in F domains. We tested the organization of chromosomal domains by measuring the relaxation-mediated transcription of eight insertions of a heterologous Ptccat cassette, two in each type of domain, showing that transcription depended on their chromosomal location. Moreover, transcription from the four promoters directing the five genes involved in supercoiling homeostasis, located either in U (gyrB), D (topA), or N (gyrA and parEC) domains was analyzed both in their chromosomal locations and in a replicating plasmid. Although expression from the chromosomal PgyrB and PtopA showed the expected domain regulation, their expression was down-regulated in the plasmid, which behaved as a D domain. However, both PparE and PgyrA carried their own regulatory signals, their topology-dependent expression being equivalent in the plasmid or in the chromosome. In PgyrA a DNA bend acted as a DNA supercoiling sensor. These results revealed that DNA topology functions as a general transcriptional regulator, superimposed upon other more specific regulatory mechanisms.es_ES
dc.description.sponsorshipThis study was supported by grant BIO2011–25343 from Plan Nacional de I+D+I of Ministerio de Ciencia e Innovación. CIBER Enfermedades Respiratorias is an initiative from Instituto de Salud Carlos III. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscripes_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.subject.meshBacterial Proteins es_ES
dc.subject.meshDNA, Bacterial es_ES
dc.subject.meshDNA, Superhelical es_ES
dc.subject.meshPromoter Regions, Genetic es_ES
dc.subject.meshStreptococcus pneumoniae es_ES
dc.subject.meshTranscriptome es_ES
dc.subject.meshGene Expression Regulation, Bacterial es_ES
dc.titleRole of global and local topology in the regulation of gene expression in Streptococcus pneumoniaees_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

Files in this item

Acceso Abierto

This item appears in the following Collection(s)

Show simple item record

Atribución 4.0 Internacional
This item is licensed under a: Atribución 4.0 Internacional