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dc.contributor.author | Amor Aramendia, Aranzazu | |
dc.contributor.author | Toro, Carlos | |
dc.contributor.author | Fernandez-Martinez, Amalia | |
dc.contributor.author | Baquero Mochales, Margarita | |
dc.contributor.author | Benito, Agustin | |
dc.contributor.author | Berzosa, Pedro | |
dc.date.accessioned | 2018-12-07T09:42:43Z | |
dc.date.available | 2018-12-07T09:42:43Z | |
dc.date.issued | 2012-03-30 | |
dc.identifier.citation | Malar J. 2012 Mar 30;11:100. | es_ES |
dc.identifier.issn | 1475-2875 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6775 | |
dc.description.abstract | BACKGROUND: Drug resistance is a major problem to control Plasmodium falciparum infection in endemic countries. During last decade, African countries have changed first-line treatment to artemisinin-based combinations therapy (ACT); sulphadoxine-pyrimethamine (SP) is recommended for Intermittent Preventive Therapy (IPT). Molecular markers related to P falciparum resistance were analysed for the period of transition from SP to ACT, in isolates imported from Africa. METHODS: A first group of samples was taken in the period between June 2002 and June 2006 (n = 113); a second group in the period between November 2008 and August 2010 (n = 46). Several alleles were analysed by nested PCR-RFLP: 51, 59, 108, 164, in the pfdhfr gene; 436, 437, 540, 581, in the pfdhps gene; 86, 1246, in the pfmdr1 gene and 76, in the pfcrt gene. The prevalence of alleles in the groups was compared with the chi-squared or Fisher's exact tests. RESULTS: The pfdhfr N51I, C59R and S108N were over to 90% in the two groups; all samples had the I164. In the pfdhps, 437 G and 581 G, increased up to 80% and 10.9% (p = 0.024), respectively in the second group. The 540 G decreases (24% to 16.%) and the 436A disappears at the end of the follow-up (p = 0.004) in the second group. The 76I-pfcrt stayed over 95% in the two groups. Prevalence of 86Y-pfmdr1 decreased over eight years. CONCLUSIONS: Pharmacological pressure affects the resistance strains prevalence. As for SP, the disappearance of 436A and the decrease in 540 G suggest that these mutations are not fixed. On the other hand, studies carried out after ACT introduction show there was a selection of strains carrying the SNPs N86Y, D1246Y in pfmdr1. In this work, the prevalence of pfmdr1- D1246Y is increasing, perhaps as a result of selective pressure by ACT. Continued surveillance is essential to monitor the effectiveness of treatments. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central (BMC) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0/ | * |
dc.subject.mesh | Africa | es_ES |
dc.subject.mesh | Alleles | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Antimalarials | es_ES |
dc.subject.mesh | Artemisinins | es_ES |
dc.subject.mesh | DNA, Protozoan | es_ES |
dc.subject.mesh | Drug Combinations | es_ES |
dc.subject.mesh | Gene Frequency | es_ES |
dc.subject.mesh | Genotype | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Malaria, Falciparum | es_ES |
dc.subject.mesh | Multidrug Resistance-Associated Proteins | es_ES |
dc.subject.mesh | Mutant Proteins | es_ES |
dc.subject.mesh | Mutation, Missense | es_ES |
dc.subject.mesh | Peptide Synthases | es_ES |
dc.subject.mesh | Plasmodium falciparum | es_ES |
dc.subject.mesh | Polymerase Chain Reaction | es_ES |
dc.subject.mesh | Polymorphism, Restriction Fragment Length | es_ES |
dc.subject.mesh | Pyrimethamine | es_ES |
dc.subject.mesh | Sulfadoxine | es_ES |
dc.subject.mesh | Tetrahydrofolate Dehydrogenase | es_ES |
dc.subject.mesh | Drug Resistance | es_ES |
dc.subject.mesh | Travel | es_ES |
dc.title | Molecular markers in plasmodium falciparum linked to resistance to anti-malarial drugs in samples imported from Africa over an eight-year period (2002-2010): impact of the introduction of artemisinin combination therapy | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 2.0 | * |
dc.identifier.pubmedID | 22462737 | es_ES |
dc.format.volume | 11 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 100 | es_ES |
dc.identifier.doi | 10.1186/1475-2875-11-100 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1475-2875 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1186/1475-2875-11-100 | es_ES |
dc.identifier.journal | Malaria journal | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Medicina Tropical | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |