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dc.contributor.authorAmor Aramendia, Aranzazu 
dc.contributor.authorToro, Carlos
dc.contributor.authorFernandez-Martinez, Amalia 
dc.contributor.authorBaquero Mochales, Margarita 
dc.contributor.authorBenito, Agustin 
dc.contributor.authorBerzosa, Pedro 
dc.date.accessioned2018-12-07T09:42:43Z
dc.date.available2018-12-07T09:42:43Z
dc.date.issued2012-03-30
dc.identifier.citationMalar J. 2012 Mar 30;11:100.es_ES
dc.identifier.issn1475-2875es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6775
dc.description.abstractBACKGROUND: Drug resistance is a major problem to control Plasmodium falciparum infection in endemic countries. During last decade, African countries have changed first-line treatment to artemisinin-based combinations therapy (ACT); sulphadoxine-pyrimethamine (SP) is recommended for Intermittent Preventive Therapy (IPT). Molecular markers related to P falciparum resistance were analysed for the period of transition from SP to ACT, in isolates imported from Africa. METHODS: A first group of samples was taken in the period between June 2002 and June 2006 (n = 113); a second group in the period between November 2008 and August 2010 (n = 46). Several alleles were analysed by nested PCR-RFLP: 51, 59, 108, 164, in the pfdhfr gene; 436, 437, 540, 581, in the pfdhps gene; 86, 1246, in the pfmdr1 gene and 76, in the pfcrt gene. The prevalence of alleles in the groups was compared with the chi-squared or Fisher's exact tests. RESULTS: The pfdhfr N51I, C59R and S108N were over to 90% in the two groups; all samples had the I164. In the pfdhps, 437 G and 581 G, increased up to 80% and 10.9% (p = 0.024), respectively in the second group. The 540 G decreases (24% to 16.%) and the 436A disappears at the end of the follow-up (p = 0.004) in the second group. The 76I-pfcrt stayed over 95% in the two groups. Prevalence of 86Y-pfmdr1 decreased over eight years. CONCLUSIONS: Pharmacological pressure affects the resistance strains prevalence. As for SP, the disappearance of 436A and the decrease in 540 G suggest that these mutations are not fixed. On the other hand, studies carried out after ACT introduction show there was a selection of strains carrying the SNPs N86Y, D1246Y in pfmdr1. In this work, the prevalence of pfmdr1- D1246Y is increasing, perhaps as a result of selective pressure by ACT. Continued surveillance is essential to monitor the effectiveness of treatments.es_ES
dc.language.isoenges_ES
dc.publisherBioMed Central (BMC) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/*
dc.subject.meshAfrica es_ES
dc.subject.meshAlleles es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAntimalarials es_ES
dc.subject.meshArtemisinins es_ES
dc.subject.meshDNA, Protozoan es_ES
dc.subject.meshDrug Combinations es_ES
dc.subject.meshGene Frequency es_ES
dc.subject.meshGenotype es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMalaria, Falciparum es_ES
dc.subject.meshMultidrug Resistance-Associated Proteins es_ES
dc.subject.meshMutant Proteins es_ES
dc.subject.meshMutation, Missense es_ES
dc.subject.meshPeptide Synthases es_ES
dc.subject.meshPlasmodium falciparum es_ES
dc.subject.meshPolymerase Chain Reaction es_ES
dc.subject.meshPolymorphism, Restriction Fragment Lengthes_ES
dc.subject.meshPyrimethamine es_ES
dc.subject.meshSulfadoxine es_ES
dc.subject.meshTetrahydrofolate Dehydrogenase es_ES
dc.subject.meshDrug Resistance es_ES
dc.subject.meshTravel es_ES
dc.titleMolecular markers in plasmodium falciparum linked to resistance to anti-malarial drugs in samples imported from Africa over an eight-year period (2002-2010): impact of the introduction of artemisinin combination therapyes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 2.0*
dc.identifier.pubmedID22462737es_ES
dc.format.volume11es_ES
dc.format.number1es_ES
dc.format.page100es_ES
dc.identifier.doi10.1186/1475-2875-11-100es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1475-2875es_ES
dc.relation.publisherversionhttps://doi.org/10.1186/1475-2875-11-100es_ES
dc.identifier.journalMalaria journales_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES


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