Mostrar el registro sencillo del ítem

dc.contributor.authorMartin-Martin, Ines 
dc.contributor.authorMolina, Ricardo 
dc.contributor.authorJimenez, Maribel 
dc.date.accessioned2018-12-05T18:43:06Z
dc.date.available2018-12-05T18:43:06Z
dc.date.issued2015-11-16
dc.identifier.citationPLoS One. 2015 Nov 16;10(11):e0140722es_ES
dc.identifier.issn1932-6203es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6770
dc.description.abstractBACKGROUND: Sand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop cellular and humoral responses against sand fly saliva. A positive correlation has been observed between the number of bites and antibody levels indicating that anti-saliva antibody response can be used as marker of exposure to sand flies. Little is known about kinetics of antibodies against Phlebotomus perniciosus salivary gland homogenate (SGH) or recombinant salivary proteins (rSP). This work focused on the study of anti-P. perniciosus saliva antibodies in sera of mice and rabbits that were experimentally exposed to the bites of uninfected sand flies. METHODOLOGY/PRINCIPAL FINDINGS: Anti-saliva antibodies were evaluated by ELISA and Western blot. In addition, antibody levels against two P. perniciosus rSP, apyrase rSP01B and D7 related protein rSP04 were determined in mice sera. Anti-saliva antibody levels increased along the immunizations and correlated with the number of sand fly bites. Anti-SGH antibody levels were detected in sera of mice five weeks after exposure, and persisted for at least three months. Anti-apyrase rSP01B antibodies followed similar kinetic responses than anti-SGH antibodies while rSP04 showed a delayed response and exhibited a greater variability among sera of immunized mice. In rabbits, anti-saliva antibodies appeared after the second week of exposure and IgG antibodies persisted at high levels, even 7 months post-exposure. CONCLUSIONS/SIGNIFICANCE: Our results contributed to increase the knowledge on the type of immune response P. perniciosus saliva and individual proteins elicited highlighting the use of rSP01B as an epidemiological marker of exposure. Anti-saliva kinetics in sera of experimentally bitten rabbits were studied for the first time. Results with rabbit model provided useful information for a better understanding of the anti-saliva antibody levels found in wild leporids in the human leishmaniasis focus in the Madrid region, Spain.es_ES
dc.description.sponsorshipThis study was partially funded by the Spanish Ministry of Science & Innovation (Project AGL2008-01592) and by the EU grant FP7-2011-261504 EDENext. The paper is catalogued by the EDENext Steering Committee as EDENext371 (http://www.edenext.eu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnimals es_ES
dc.subject.meshAntibodies es_ES
dc.subject.meshBlotting, Westernes_ES
dc.subject.meshDisease Models, Animales_ES
dc.subject.meshEnzyme-Linked Immunosorbent Assay es_ES
dc.subject.meshFemale es_ES
dc.subject.meshImmunoglobulin G es_ES
dc.subject.meshLeishmania es_ES
dc.subject.meshLeishmaniasis es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred BALB C es_ES
dc.subject.meshPhlebotomus es_ES
dc.subject.meshRabbits es_ES
dc.subject.meshRecombinant Proteins es_ES
dc.subject.meshSaliva es_ES
dc.subject.meshSalivary Glands es_ES
dc.subject.meshSalivary Proteins and Peptides es_ES
dc.subject.meshSpain es_ES
dc.titleKinetics of Anti-Phlebotomus perniciosus Saliva Antibodies in Experimentally Bitten Mice and Rabbitses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID26569103es_ES
dc.format.volume10es_ES
dc.format.number11es_ES
dc.format.pagee0140722es_ES
dc.identifier.doi10.1371/journal.pone.0140722es_ES
dc.contributor.funderUnión Europea. Comisión Europea. 7 Programa Marco 
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1932-6203es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0140722es_ES
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/261504/es_ES
dc.rights.accessRightsopen accesses_ES


Ficheros en el ítem

Acceso Abierto
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional