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dc.contributor.author | Alari-Pahissa, Elisenda | |
dc.contributor.author | Notario, Laura | |
dc.contributor.author | Lorente, Elena | |
dc.contributor.author | Vega-Ramos, Javier | |
dc.contributor.author | Justel, Ana | |
dc.contributor.author | Lopez, Daniel | |
dc.contributor.author | Villadangos, José A | |
dc.contributor.author | Lauzurica, Pilar | |
dc.date.accessioned | 2018-11-22T12:21:25Z | |
dc.date.available | 2018-11-22T12:21:25Z | |
dc.date.issued | 2012-10-30 | |
dc.identifier.citation | PLoS One. 2012;7(10):e48593 | es_ES |
dc.identifier.issn | 1932-6203 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6703 | |
dc.description.abstract | CD69 is rapidly upregulated on T cells upon activation. In this work we show that this is also the case for CD69 expression on dendritic cells (DC). Thus, the expression kinetics of CD69 on both cell types is reminiscent of the one of costimulatory molecules. Using mouse models of transgenic T cells, we aimed at evaluating the effect of monoclonal antibody (MAb)-based targeting and gene deficiency of CD69 expressed by either DC or T cells on the extent of antigen (Ag)-specific T cell priming, which could be the result of a putative role in costimulation as well as on DC maturation and Ag-processing and presentation. CD69 targeting or deficiency of DC did not affect their expression of costimulatory molecules nor their capacity to induce Ag-specific T cell proliferation in in vitro assays. Also, CD69 targeting or deficiency of transgenic T cells did not affect the minimal proliferative dose for different peptide agonists in vitro. In in vivo models of transgenic T cell transfer and local Ag injection, CD69 deficiency of transferred T cells did not affect the extent of the proliferative response in Ag-draining lymph nodes (LN). In agreement with these results, CD69 MAb targeting or gene deficiency of Vaccinia-virus (VACV) infected mice did not affect the endogenous formation of virus-specific CD8(+) T cell populations at the peak of the primary immune response. Altogether our results argue against a possible role in costimulation or an effect on Ag processing and presentation for CD69. | es_ES |
dc.description.sponsorship | E. Alari-Pahissa was supported by predoctoral fellowships from the Spanish Ministry of Education and Science. This work was supported by grants (SAF2007-64310 and SAF2010-15649) from the Ministerio de Educación, Ciencia y Tecnología and La Fundació La Marató de TV3 2004. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (PLOS) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Adoptive Transfer | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Antigen Presentation | es_ES |
dc.subject.mesh | Antigens | es_ES |
dc.subject.mesh | Antigens, CD | es_ES |
dc.subject.mesh | Antigens, Differentiation, T-Lymphocyte | es_ES |
dc.subject.mesh | CD8-Positive T-Lymphocytes | es_ES |
dc.subject.mesh | Cell Proliferation | es_ES |
dc.subject.mesh | Cells, Cultured | es_ES |
dc.subject.mesh | Dendritic Cells | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Flow Cytometry | es_ES |
dc.subject.mesh | Lectins, C-Type | es_ES |
dc.subject.mesh | Lipopolysaccharides | es_ES |
dc.subject.mesh | Lymph Nodes | es_ES |
dc.subject.mesh | Lymphocyte Activation | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mice, Inbred BALB C | es_ES |
dc.subject.mesh | Mice, Inbred C57BL | es_ES |
dc.subject.mesh | Mice, Knockout | es_ES |
dc.subject.mesh | Mice, Transgenic | es_ES |
dc.subject.mesh | Oligodeoxyribonucleotides | es_ES |
dc.subject.mesh | T-Lymphocytes | es_ES |
dc.subject.mesh | Up-Regulation | es_ES |
dc.subject.mesh | Vaccinia | es_ES |
dc.subject.mesh | Vaccinia virus | es_ES |
dc.title | CD69 does not affect the extent of T cell priming | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 23119065 | es_ES |
dc.format.volume | 7 | es_ES |
dc.format.number | 10 | es_ES |
dc.format.page | e48593 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0048593 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1932-6203 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0048593 | es_ES |
dc.identifier.journal | PloS one | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2007-64310 | es_ES |
dc.rights.accessRights | open access | es_ES |